E16.5d胚胎小鼠皮肤细胞作为毛囊重建模型种子细胞的可行性研究  

Feasibility of follicle reconstitution with embryonic 16.5 d fetal mouse skin cells as seed cells

作　　者：夏学颖[1] 何伟 孙赛[1] 张碧芳[1] 李丽燕[1] 陈容容[1] 姜金豆[1] 胡葵葵[1] 

机构地区：[1]广东省妇幼保健院医学美容科,广州510010 [2]顺德区第一人民医院烧伤整形科

出　　处：《山西医科大学学报》2017年第2期129-131,共3页Journal of Shanxi Medical University

基　　金：广东省自然科学基金资助项目(2014A030313675);广州市科技计划基金资助项目(2014J4100139)

摘　　要：目的研究胚胎小鼠皮肤及其毛囊的发育规律,探讨胚胎小鼠的皮肤细胞作为毛囊重建模型种子细胞的可行性。方法对80只胎龄14.5-18.5 d小鼠及新出生的小鼠皮肤进行连续石蜡切片,连续切片进行HE染色,观察其组织学结构并了解其附件形成情况。免疫荧光染色观察胚胎表皮特异性蛋白细胞角蛋白8(cytokeratin 8,CK8)和18(CK18)在胚胎小鼠皮肤表达。结果胎龄14.5 d(embryonic day 14.5,E14.5 d)的胚胎小鼠,表皮层形成表皮细胞仅为1-2层;E16.5 d的胎鼠背部皮肤较薄,表面凹凸不平并形成褶皱,未见毛囊形成;E18.5 d胎鼠毛囊发育达到旺盛的时期,毛囊数量明显增加;新出生的小鼠皮肤附件基本发育成熟。E16.5 d小鼠皮肤中表皮干细胞标记物CK8和CK18为阳性。结论 16.5 d胚胎小鼠(E16.5)的皮肤细胞可以作为毛囊重建模型的种子细胞。Objective To investigate the periodical changes of hair follicles and explore the feasibility of constructing follicle reconsti- tution model with embryonic skin cells. Methods The skin structures of embryonic 14.5 - 18.5 d fetal mice and neonatal（1-0） mice were observed by HE staining, and the expression of CK8/18 was observed in skin using immunofluorescence. Results Only 1 -2 layers of keratinocytes was seen in the El4.5 d fetal mouse. The hair follicles were not found in the El6.5 d fetal mouse. The skin be- came thicker gradually in the El8.5 d fetal mouse, and the skin appendage development basically completed. Skin appendages were mature in neonatal mice. The expression of CK8/18 was positive in the El6.5 d fetal mouse. Condusion El6.5 d fetal mouse skin cells can be used as seed cells in follicle reconstitution.

关 键 词：种子细胞 毛囊 表皮干细胞 细胞角蛋白 胚胎小鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]