光遗传诱发神经元单位放电的实验教学设计与思考  

The design and experience of experiment teaching in neuronal discharge evoked by the optogenetics

作　　者：吴广延[1] 孙琳[2] 何超[1] 

机构地区：[1]第三军医大学基础部生理学教研室,重庆400038 [2]西南大学体育学院,重庆400715

出　　处：《中华医学教育探索杂志》2017年第1期53-55,共3页Chinese Journal of Medical Education Research

基　　金：国家自然科学基金青年项目（31500862）

摘　　要：神经元单位放电的记录与分析是基础医学综合实验的重要内容之一，但传统实验并不能很好使学生理解神经元动作电位的产生机制。结合八年制医学生综合素质高、培养要求高等特点．授课教师将前沿技术——光遗传学技术融合到该实验中，开设了“光遗传诱发神经元单位放电”实验。在此，对“光遗传诱发神经元单位放电”的教学设计作了详细介绍，并总结教学经验、提出自身思考。The recording and analysis for the neurons＇ single unit discharge is one of the important content among the comprehensive experiments in basic medicine. However, the traditional experiment can- not help the students to understand the mechanism of the action potential. According to the high compre- hensive quality and high requirements of training in eight-year medical students, in the past two years, we have entrained the optogenetics into the single unit recording experiment and set up an experiment of neuronal discharge evoked by the optogenetics＂. In this article, we the introduce teaching design of the recording and analysis of the unit discharges induced by light genetics, sum up teaching experience and put forward our own thinking.

关 键 词：光遗传 神经元 单位放电 细胞外记录 

分 类 号：R68[医药卫生—骨科学]