气相色谱法测定薄荷脑鼻用原位凝胶喷雾剂中薄荷脑的含量  

Determination of the content of menthol in the nasal in-situ gel spray by gas chromatography

作　　者：康安锋 李新方[2] 乐李敬 詹洁琼 马志强[2] 张欣荣[2] 杨峰[1,2] 

机构地区：[1]福建中医药大学,福州350122 [2]第二军医大学药学院无机化学教研室 [3]河北北方学院研究生部

出　　处：《海军医学杂志》2017年第1期24-26,共3页Journal of Navy Medicine

基　　金：全军后勤科研计划重大项目(AWS14L005)

摘　　要：目的建立薄荷脑鼻用原位凝胶喷雾剂中薄荷脑含量的测定方法。方法采用气相色谱法测定。色谱柱为Agilent DB-WAX毛细管柱(30 m×0.25 mm×0.25μm),柱温为120℃,检测器为氢火焰离子化检测器,检测器温度为260℃,进样口温度为220℃,进样量为1μl,载气为氮气,柱流速为1.0 ml/min。结果薄荷脑质量浓度在0.02~0.20 mg/ml范围内具有良好的线性关系(r=0.999 1);精密度、重复性、稳定性试验的RSD<2%;加样回收率为95.1%~103.4%,RSD为2.3%(n=6)。结论该方法操作简便、结果可靠、重复性好,可用于测定薄荷脑鼻用原位凝胶喷雾剂中薄荷脑含量。Objective To establish a method for concentration determination of menthol in the nasal in -situ gel spray. Methods Gas chromatography （GC） was performed on the column of Agilent DB-WAX capillary column （30m ×0.25 mm ×0.25μm）, with the column temperature of 120 ℃.With nitrogen gas used as carrier gas , hydrogen flame ion detector （FID） was used, the detection temperature was 260℃, injection temperature was 220℃, injection volume was 1μl and column flow rate was 1.0 ml/min. Results For menthol, there was good linear relationship within the range of mass concentration at 0.02~0.20 mg/mL （r=0.9991）. Precision, reproducibility and stability test were all lower than 2%, the sample recovery rate was 95.1%~103.4%and relative stand-ard deviation （RSD） was 2.3%（n=6）.Conclusion The established method had the following advantages：easy operation, high re-liability and good reproducibility , and it could be used for the content determination of menthol in the nasal in -situ gel spray .

关 键 词：气相色谱法 原位凝胶 薄荷脑 含量测定 

分 类 号：R917[医药卫生—药物分析学]