内脂素和L-PGDS与下肢动脉硬化的相关性研究  

Correlation of visfatin and L-PGDS with lower extremity arteriosclerosis

作　　者：肖磊[1] 李俊海[1] 张秀军[1] 

机构地区：[1]天津医科大学

出　　处：《中国生化药物杂志》2017年第1期298-301,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的 探讨内脂素与脂质运载蛋白型前列腺素D合酶（lipocalintype prostaglandin dsynthase,L-PGDS）在下肢动脉粥样硬化斑块中的关系。方法 收集2014年2月~2016年2月在天津医院血管外科行股动脉粥样硬化斑块剥脱术中获取的内膜标本40例（观察组）,20例脾动脉、肠系膜动脉标本（对照组）,采用免疫组化染色法测定各组内脂素和L-PGDS蛋白表达,逆转录-聚合酶链反应（reverse transcriptionpolymerase chain reaction,RT-PCR）测定各组内脂素和L-PGDS m RNA表达。结果 观察组内脂素蛋白表达为（121.42±11.07）,明显高于对照组（72.07±12.81）,而L-PGDS为（87.93±9.73）,明显低于对照组（107.04±10.58）,差异比较有统计学意义（P〈0.05）;观察组内脂素mRNA相对表达量为（0.321±0.024）,明显高于对照组（0.217±0.031）,而L-PGDS m RNA为（0.203±0.018）,明显低于对照组（0.314±0.029）,差异比较有统计学意义（P〈0.05）;斑块组织内脂素蛋白与L-PGDS蛋白表达呈负相关（r=-0.617,P〈0.05）,内脂素m RNA和L-PGDS m RNA同样呈负相关（r=-0.645,P〈0.05）。结论 内脂素和L-PGDS与下肢动脉粥样硬化发生、发展有重要关系,两者可能起到相互拮抗的作用。Objective To investigate the relationship between lipocalintype prostaglandin dsynthase（L-PGDS） and visfatin in the lower extremity atherosclerotic plaque. Methods Collected from February 2014 to February 2016 in vascular surgery of our hospital 40 cases of femoral artery atherosclerotic plaque intima specimens （observation group）, 20 cases of splenic artery, superior mesenteric artery samples （control groiqj）, the expression of visfatin and L-PGDS protein were detected by immunohistochemical staining, and tiie expression of visfetin and L-PGDS mRNA were determined by RT- PCR. Results The observation groiq） visfatin protein expression was （121.42±11.07）, significantly higher than the control group （72.07±12.81）, and L-PGDS protein expression was （87.93±9.73）, significantly lower than the control group （107.04±10.58）, the difference was statistically significant （P〈0.05）. The relative expression of visfatin mRNA in the observation group was （0.321±0.024）, which was significant higher than that of the control group （0.217±0.031）, while L-PGDS mRNA was （0.203±0.018）, significantly lower than the control groiq〉 （0.314±0.029）, the difference was statistically significant （P〈0.05）; The expression of L-PGDS protein was negatively correlated with the expression of visfetin protein in the plaque tissue （r=-0.617, P〈0.05）, visfatin mRNA and L-PGDS mRNA were negatively correlated （r=-0.645, P〈 0.05）. Conclusion sfatin and L-PGDS in lower extremity atherosclerosis occurrence and development have an important relationship, both of them may play an antagonistic role.

关 键 词：动脉粥样硬化 内脂素 L-PGDS 相关性 

分 类 号：R543.12[医药卫生—心血管疾病]