水蛭素提取纯化研究进展  

Research progress of extraction and purification of Hirudin

作　　者：黄胜[1] 熊伟[1] 姚俊杰[1] 李路[1] 陈红雕 杨引欢 姚洪君 

机构地区：[1]贵州大学动物科学学院

出　　处：《中国生化药物杂志》2017年第2期313-315,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：贵州大学大学生SRT项目[贵大SRT字(2015)];水产养殖特色专业项目[(801136110201)和095号];贵州省特色水产产业技术体系项目;贵州省黔西南特色产业扶持与生态修复协同创新中心平台建设项目

摘　　要：水蛭素（Hirudin）是水蛭唾液腺分泌物中的一种蛋白,是迄今为止发现的最强的天然凝血酶特效抑制剂,具有活血、化淤和通经作用。但水蛭躯体中存在无抗凝活性的伪水蛭素组分和分泌物中大量成分复杂的物质,增加了水蛭素分离纯化难度;临床运用对生物制品的纯度要求高,世界各地很多科学家对水蛭素的提取和纯化做了许多相关的研究。因此,本综述根据相关研究,对水蛭素的提取、纯化工作进行介绍,分析各种提取及纯化方法的利弊,展望其发展趋势,以期为今后水蛭素的提取纯化提供借鉴。Hirudin as a novel anticoagulant and antithromotic agent,is a kind of protein that is obtained from the salivary gland secretion of one kind blood-sucking leech.It increases the difficulty extraction and purification of hirudin that composition of no anticoagulant activity of peseudo-hirudin in the body of leech and a large number of complex material from the salivary gland secretion of one kind blood-sucking leech.This article focuses on reviewing the structure of hirudin,genetic makeup,extraction and purification of hirudin according to the related research.Analysis of the pros and cons of various extraction and purification methods.Looking forward to its developing tendency,so as to provide reference for extraction and purification of Hirudin.

关 键 词：水蛭素 提取 纯化 

分 类 号：Q516[生物学—生物化学] TQ464[化学工程—制药化工]