HPLC法测定藏药3种甘扎嘎日药材中金丝桃苷的含量  

Determination of hyperoside in 3 Tibetan medicine Kandrakaris by HPLC

作　　者：热增才旦[1] 利毛才让[2] 陈晓红[1] 童丽[1] 李文渊[1] 杨芳[1] 

机构地区：[1]青海大学医学院中藏药研究中心,西宁810001 [2]青海师范大学民师院化学系,西宁810008

出　　处：《西北药学杂志》2017年第2期127-130,共4页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81260684;81160469);教育部"春晖计划"项目(编号:Z2014025)

摘　　要：目的建立甘扎嘎日药材秀丽莓、红泡刺藤和库页悬钩子中有效成分金丝桃苷的含量测定方法。方法采用HPLC法。在Agela Durashell C_(18)(250mm×4.6mm,5μm)色谱柱上,以乙腈-甲醇-4mL·L^(-1)磷酸水溶液为流动相,梯度洗脱,检测波长为368nm,柱温为25℃。结果金丝桃苷质量浓度在0.004 0~0.064 0mg·mL^(-1)(r=0.999 6)范围内呈良好的线性关系,平均加样回收率为97.35%(RSD=1.09%)。结论该方法操作简便,结果准确、稳定,重复性好,可作为甘扎嘎日药材质量控制和评价的有效方法。Objective To establish an HPLC method for the determination of hyperoside in Kandrakaris such as Rubus amabilis Focke,Rbus niveus Thunb.and Rubus sachalinensia Leveille.Methods The separation was performed on an Agela Durashell C（18）（250mm×4.6mm,5μm）column with a mobile phase consisting of acetonitrile-methanol-water containing 4mL·L-（-1） phosphate in gradient elution.The detection wavelength was set at 368 nm,and the column temperature was 25 ℃.Results The calibration curves were linear within the range of 0.004 0-0.064 0 mg·mL^-1（r=0.999 6）for hyperoside.The average recovery was97.35%（RSD=1.09%）.Conclusion The established method for hyperoside in Kandrakarisis simple,the result is accurate,stable and reproducible,and it is acceptable for the quality assurance and evaluation of Kandrakaris.

关 键 词：金丝桃苷 藏药 甘扎嘎日 HPLC 含量 

分 类 号：R282[医药卫生—中药学]