温和灸对瘙痒模型小鼠行为学及亚细胞形态的影响  

Effects of warming moxibustion on behavioristics and cell morphology in pruritus mice

作　　者：徐利[1] 林国强[1] 葛琼翔[1] 

机构地区：[1]浙江中医药大学附属第一医院肛肠外科,浙江杭州310006

出　　处：《预防医学》2017年第3期236-239,共4页CHINA PREVENTIVE MEDICINE JOURNAL

基　　金：浙江省自然科学基金(Q15H270016)

摘　　要：目的观察不同温和灸对瘙痒模型小鼠行为学及亚细胞形态的影响。方法将40只C57BL/6J小鼠随机分为空白组、模型组、治疗I组(38℃艾灸)和治疗Ⅱ组(46℃艾灸)4组,每组10只。采用背部皮下注射辣椒素法造模。造模成功后,选取治疗I、Ⅱ组小鼠"长强穴""神阙穴"艾灸治疗一周。采用面颊部注射组胺法观察小鼠1 min内面颊部搔抓次数,并比较4组小鼠搔抓次数差异,分析温和灸治疗瘙痒症效果。透射电子显微镜(TEM)观察小鼠面颊和肛周皮肤细胞中液泡数量,分析温和灸对瘙痒模型小鼠亚细胞形态的影响。结果面颊部注射组胺后,4组小鼠1 min内面颊部搔抓次数比较,差异有统计学意义(P<0.01),两两比较结果显示,治疗I组搔抓(9.4±1.7)次和治疗Ⅱ组(8.3±1.2)次均少于模型组的(11.6±2.3)次(P<0.01)。电镜结果显示4组小鼠面颊皮肤中液泡数量差异无统计学意义(P>0.05),但肛周皮肤中液泡数量差异有统计学意义(P<0.05)。与模型组小鼠肛周皮肤实质细胞中液泡数量(30.00±9.17)比较,治疗I组(17.33±5.51)和治疗Ⅱ组(5.67±2.08)均减少(P<0.05);与治疗I组比较,治疗Ⅱ组小鼠肛周皮肤细胞中液泡数量减少(P<0.05)。两两比较结果显示,治疗I、Ⅱ组肛周皮肤细胞中液泡数量少于面颊部皮肤细胞(P<0.05)。结论温和灸对瘙痒模型小鼠的瘙痒症状具有明显的改善作用,可能与长期温和灸刺激减少皮肤细胞中的液泡数量有关。Objective To explore the effects of warming moxibustion on behavioristics and cell morphology in pruritus mice. Methods Forty C57BL/6J mice were randomly divided into control group, model group, treatment group I and treatment group II. Mice were injected subcutaneously with vanilloid on the back to establish itch model. Treatment group I and group II were given moxibustion treatment at Changqiang （ GV1 ） and Shenque （ CV8 ） . After one week treatment, histamine was injected into the cheek to observe the itching behavior, and the skin of cheek and crissum was observed under the transmission electron microscope to explore the effects of moxibustion on cell morphology. Results Scratching behavior and the number of vacuole in the perianal cells among four groups were significantly different. Scratching behavior reduced in the treatment group 1（9. 4 ±1.7） and treatment group II （ 8. 3 ± 1. 2 ） compared with the model group （11. 6 ± 2. 3 ） . The number of vacuole were also reduced in the treatment group I （17. 33 ±5. 51） and treatment group II （5. 67 ± 2. 08） compared with the model group （ 30. 00 ± 9. 17）. In treatment group I and II, the number of vacuole in the perianal cells significantly reduced compared with cells in the skin of cheek. Conclusion Warming moxibustion has a potent depressant effect on histamine - induced scratching behavior and the decrease number of vacuole may be one of the mechanisms.

关 键 词：温和灸 瘙痒症 辣椒素 组胺 

分 类 号：R246[医药卫生—针灸推拿学]