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作 者:李书光[1,2] 王玉茂[2] 刘吉山[2] 程立坤[1,2] 尹梅芳 张娜[2] 赵家磊 沈志强[2]
机构地区:[1]山东省滨州畜禽蜂胶疫苗研究开发推广中心,山东滨州256600 [2]山东省滨州畜牧兽医研究院,山东滨州256600 [3]滨州市滨城区畜牧兽医局,山东滨州256600
出 处:《家畜生态学报》2017年第2期64-66,共3页Journal of Domestic Animal Ecology
基 金:山东省自然科学科学基金青年基金项目(ZR2014CQ009);山东省现代农业产业技术体系牛产业创新团队(SDAIT-09-12)
摘 要:近年来,山东省常发生类似牛传染性胸膜肺炎症状的疾病。通过病料的采集、支原体分离、生化鉴定、PCR检测、16SRNA序列分析及临床治疗等研究工作,证实成功分离到一株牛支原体,且能够形成典型的煎蛋状的菌落;分子生物学检测进一步确定为牛支原体,临床上使用疫苗和敏感药物,能够迅速控制疫情的发生。这是牛支原体病在山东省的首次发病报道,有利于下一步该病科学防控。In recent years, suspected bovine contagious pleural pneumonia disease often occured in Shandong. A successful isolation and identification of one strain mycoplasma bovis was carried out by the collection of the disease, Mycoplasma isolation, biochemical identification, PCR, 16sRNA sequence analy-sis and clinical treatment and other research work. In the solid medium, the isolates were grown as typical colonies in the shape of a fried egg. In clinical practice, the use of vaccines and sensitive drugs can quickly control the epidemic situation of cattle herds. This is the first reported case of bovine mycoplasma disease in Shandong region, its diagnosis and treatment process is conducive to the further prevention and control of the disease.
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