扶正抗艾颗粒对HIV感染者外周血树突状细胞亚群的影响  

Effect of Fuzheng Kangai Granule on Dendritic Cell Subsets in Peripheral Blood of HIV Infection

作　　者：肖明中[1] 刘建忠[1] 陈瑶[2] 

机构地区：[1]湖北省中医院,湖北武汉430061 [2]湖北中医药大学,湖北武汉430065

出　　处：《中医学报》2017年第2期159-162,共4页Acta Chinese Medicine

基　　金：国家中医临床研究基地业务建设科研专项课题{国中医药科[2012]39号JDZX2012024}

摘　　要：目的:观察扶正抗艾颗粒对人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者外周血树突状细胞(dendritic cell,DC)亚群的影响。方法:选取15例未接受过高效抗逆转录病毒(highly active antiretroviral treatment,HAART)治疗和16例HAART治疗后(免疫重建不全)的HIV感染者,随机分为两组,中药治疗组予以单纯中药扶正抗艾颗粒治疗,中药联合HAART治疗组予以HAART抗病毒+扶正抗艾颗粒联合治疗,10例健康人作为树突状细胞亚群的基线对照,观察治疗前后HIV感染者症状、体征积分变化,并采用流式细胞技术检测患者外周血CD4+细胞亚群计数以及DC亚群的变化情况。结果:与治疗前比较,15例中药治疗组患者症状、体征积分显著下降(P<0.01),乏力积分显著减少(P<0.01),气短、纳呆积分显著减少(P<0.05)。CD4+T淋巴细胞有上升趋势,但治疗前后比较差异无统计学意义(P>0.05)。浆样DC(plasmacytoid DC,PDC)频率及髓样DC(myeloid DC,MDC)频率均值较治疗前增高,其中MDC频率较治疗前差异有统计学意义(P<0.05),PDC频率无显著差异。与治疗前比较,16例联合用药患者症状、体征积分下降,差异有统计学意义(P<0.01),乏力积分显著减少(P<0.01),气短、自汗积分显著减少(P<0.05)。CD4+T淋巴细胞计数、CD8+T淋巴细胞计数及CD4+/CD8+比值治疗前后比较,差异有统计学意义(P<0.05),PDC频率及MDC频率治疗前后比较,差异有统计学意义(P<0.01)。结论:扶正抗艾颗粒可以改善患者临床症状、影响CD细胞亚群数量和功能,提高HIV感染者免疫功能。Objective： To observe the effect of Fuzheng Kangai Granule on dendritic cell in subsets the peripheral blood of patients with human immunodeficiency virus （HIV）infection. Methods： 15 cases had not received highly active antiretroviral treatment （HAART） treatment and 16 cases of HAART after treatment （immune reconstitution incompetence） HIV infection, using Chinese traditional medicine Fuzheng Kangai Granule and HAART antiviral therapy for 3 months, 10 cases of heahhy people as the baseline of dendritic cells subsets of the control. The changes of the symptom scores of HIV infection were observed before and after treat- ment, peripheral blood counts of CD4 cell subsets and changes of dendritic cell subsets were detected by flow cytometry. Results：Compared with before treatment, 15 cases of single traditional Chinese medicine treatment group with total score decreased signifi- candy （P 〈0.01 ） ,the weak integral decrease was statistically significant （P 〈0.01 ） ;shortness of breath,anorexia integral decrease was statistically significant （P 〈 0.05 ）. CD4 ~ T showed an upward trend, but there was no significant difference between before and after treatment （ P 〉 0.05 ）. Plasmacytoid DC （pDC） and myeloid DC （mDC） frequency were higher than before treatment, the fre- quency of MDC was statistically significant （ P 〈 0.05 ） ; PDC frequency was not statistically significant. Compared with before treat- ment, 16 cases of total score of combined medication were decreased, and the difference was statistically significant （P 〈 0.01 ）, the weak integral decreased was statistically significant （P 〈 0.01 ） ; shortness of breath, sweating score reduction was statistically signifi- cant （P 〈 O. 05 ）. CD4 ＋T cell count, CD8 ＋T cell count, and ratio of CD4 ＋/CDs ＋ , before and after the treatment, the difference was statistically significant （P 〈 0.05 ）, PDC frequency and MDC frequency before and after the treatment, the difference was statisti

关 键 词：HIV感染者 艾滋病 扶正抗艾颗粒 高效抗逆转录病毒疗法 树突状细胞亚群 中医药治疗 中西医结合 

分 类 号：R259.129.1[医药卫生—中西医结合]