白藜芦醇对人上皮性卵巢癌细胞增殖和凋亡的影响  

Effect of Resveratrol on the Proliferation and Apoptosis of Human Epithelial Ovarian Cancer Cells

作　　者：李研[1] 

机构地区：[1]邯郸市中心医院,河北邯郸056001

出　　处：《中医学报》2017年第2期166-170,共5页Acta Chinese Medicine

基　　金：河北省中医药管理局项目(2014008)

摘　　要：目的:研究白藜芦醇抑制人上皮性卵巢癌细胞增殖并促进其凋亡的作用及机制。方法:将人上皮性卵巢癌细胞株SKOV3细胞随机分为5组,即正常对照组、白藜芦醇高剂量组(40 mg·L^(-1))、白藜芦醇中剂量组(20 mg·L^(-1))、白藜芦醇低剂量组(10 mg·L^(-1))和顺铂组(40 mg·L^(-1))。干预48 h后,通过光学显微镜观察各组细胞形态,MTT比色法检测各组细胞增值抑制率。通过流式细胞仪分析细胞周期的改变、流式细胞术检测细胞凋亡并计算细胞凋亡率,采用RT-PCR法检测细胞Bcl-2 mRNA和Bax mRNA表达并计算Bax/Bcl-2比值,Western blot法检测细胞caspase-3蛋白表达。结果:与正常对照组比较,白藜芦醇高、中剂量组细胞出现变圆、脱壁、细胞间接触松散等状态,细胞抑制作用明显、增殖抑制率显著升高(P<0.01);白藜芦醇各剂量组细胞周期被阻滞于G2/M期,白藜芦醇高、中剂量组细胞凋亡率显著升高(P<0.01),同时可明显下调Bcl-2 mRNA而上调Bax mRNA表达,显著提高Bax/Bcl-2值(P<0.05,P<0.01),上调caspase-3蛋白表达(P<0.01)。结论:白藜芦醇能够通过抑制增殖并促进凋亡对人上皮性卵巢癌SKOV3细胞生长起到一定的抑制作用,其机制可能与白藜芦醇调节凋亡相关基因蛋白表达有关。Objective ：To investigate the effect and mechanism of resveratrol（Res） on the proliferation and apoptosis of epithelial o- varian cancer cell. Methods：The SKOV3 cells of epithelial ovarian cancer were divided randomly into five groups：normal control group, Res （40,20,10 μg · mL-1 ） groups and Cisplatin 40 μg · mL-1 group. 48 h later, the cell morphology were observed by op- tical microscope, the cellular growth inhibition rate was calculated by MTF, cell cycle and apoptosis rate were analyzed by flow cy- tometry, the expression of Bax and Bcl-2 mRNA were detected by RT-PCR, the protein level of caspase-3 was detected by Western blot. Results ： Compared with normal control group, the morphological of Res （40,20 μg · mL-1 ） groups were abnormalities, the cellular growth inhibition rate were significantly increased（P 〈 0.01 ） ,the cell cycle were arrested at G2/M phase, the apoptosis rate were significantly increased（ P 〈 0.01 ） ;the expression of bcl-2 mRNA was significantly decreased,but the expression of Bax mRNA was significantly increased, and the ratio of Bax/bcl-2 were significantly increased （ P 〈 0.05, P 〈 0.01 ）, the expression of easpase-3 protein were significantly increased（P 〈0.01 ）. Conclusion： Res could effectively inhibit epithelial ovarian cancer SK- OV3 cell proliferation and promote apoptosis, which perhaps related to its effects of regulating apoptosis related gene protein ex- pression.

关 键 词：人上皮性卵巢癌 SKOV3细胞 白藜芦醇 肿瘤 细胞增殖 细胞凋亡 中药 

分 类 号：R273.731[医药卫生—中西医结合]