脾气虚大鼠主要消化吸收结构表面血流量及血清TXA2/PGI2比值的研究  

Studies of Blood Flow in Pancreas,Small Intestine and Mesenterium and Serum TXA_2/PGI_2 Value in Spleen Qi Deficiency

作　　者：乔海峰[1,2] 刘文俊[1] 刘旭东[1] 王凌志[1] 刘慧慧[1] 王德山[1] 单德红[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁省朝阳市中心医院

出　　处：《中国医学创新》2017年第5期45-48,共4页Medical Innovation of China

基　　金：国家重点基础研究发展计划项目(973项目)(2013CB531702)

摘　　要：目的:通过检测主要消化吸收结构的表面微循环血流量及血清血栓素A2(TXA2)/前列环素I2(PGI2)(T/P)比值,从血流方面探讨脾气虚的发生机制。方法:16只SPF级雄性SD大鼠,随机分为对照组和脾气虚组(模型组),每组8只;激光散斑技术检测大鼠肝、胰腺、胃、小肠和肠系膜等表面微循环的实时血流量;ELISA法检测血清TXA2和PGI2水平。结果:与对照组相比,模型组大鼠胰腺、胃、小肠和肠系膜血流量均显著减少(P<0.01,P<0.05,P<0.05,P<0.01),血清TXA2和T/P比值也明显下降(P<0.05)。结论:脾气虚的发生与胰腺、胃、小肠和肠系膜等部位血流量减少有关,但其机制与T/P比值下降无密切关系。Objective： To study the development mechanism of Spleen Qi Deficiency in blood dynamics by detecting blood flow changes in liver, pancreas, stomach, small intestine and mesenterium, and the TXA2/PGI2 （ T/P ）value.Method： A total of 16 SPF rats were randomly divided in the control and Spleen Qi Deficiency group （ the model group ）, 8 rats in each group.Laser speckle was used to detect blood flow changes in liver, pancreas, small intestine and mesenterium.ELISA was employed to measure serum TXA2 and PGI2 concentrations.Result： Compared with the control group, blood flows in pancreas, stomach, small intestine and mesenterium in the model group were significantly reduced （P〈0.01, P〈0.05, P〈0.05, P〈0.01 ）, and serum TXA2 concentration and TXAJPGI2 value in the model group were decreased obviously （P〈0.05） .Conclusion： The reduction of the blood flow in pancreas, stomach, small intestine and mesenterium contributes to the development of Spleen Qi Deficiency, and its underlying mechanism is not related to the decrease of T/P value.

关 键 词：脾气虚 胰腺 胃 小肠 肠系膜 TXA2 PGI2 血流量 

分 类 号：R285.5[医药卫生—中药学]