健脾益肾方对老年性痴呆模型大鼠记忆能力及海马CA1区JNK蛋白表达的影响  

Effect of Jianpi Yishen Prescription on Memory Ability and Expression of JNK Protein in Hippocampal CA1 Region of Alzheimer 's Disease Model Rats

作　　者：孙岩[1] 崔书克[1] 刘锐[1] 马铭晨[1] 

机构地区：[1]河南中医药大学第三附属医院,河南郑州450008

出　　处：《中医学报》2017年第1期89-92,共4页Acta Chinese Medicine

基　　金：2013年河南省教育厅重点支持项目(13B360096)

摘　　要：目的:观察健脾益肾方对老年性痴呆模型大鼠记忆能力及海马CA1区JNK蛋白表达的影响。方法:将40只SD大鼠随机分为空白组、模型组、脑复康组、健脾益肾方组,每组10只。复制老年性痴呆大鼠模型,观察大鼠学习记忆能力及海马CA1区JNK蛋白表达的变化。结果:造模后模型组、脑复康组、健脾益肾方组大鼠Y型电迷宫测试结果分别为(25.4±4.30)次、(25.2±4.02)次、(24.1±5.51)次,显著高于空白组(P<0.05);治疗后模型组、脑复康组、健脾益肾方组大鼠Y型电迷宫测试结果分别为(23.5±4.71)次、(20.0±2.31)次、(17.0±3.16)次,脑复康组、健脾益肾方组显著低于模型组(P<0.05),健脾益肾方组显著低于脑复康组(P<0.05)。模型组、脑复康组、健脾益肾方组大鼠海马CA1区JNK蛋白阳性表达评分分别为(32.9±4.82)分、(28.6±5.32)分、(22.4±4.90)分,脑复康组、健脾益肾方组显著低于模型组(P<0.05),健脾益肾方组显著低于脑复康组(P<0.05)。结论:健脾益肾方可以改善AD大鼠的学习记忆能力,其机制可能与海马CA1区JNK蛋白表达降低有关。Objective ： To observe the effect of Jianpi Yishen Decoction on the memory ability and the expression of JNK protein in hippocampal CA1 region of Alzheimer＇s Disease model rats. Methods：Forty SD rats were randomly divided into blank group, mod- el group, Naofukang group and Jianpi Yishen Prescription group. The learning and memory ability of rats and the expression of JNK protein in hippocampal CA1 region were observed in rats. Results：The resuhs of Y-type electric maze test of model group, Naofu- kang group and Jianpi Yishen Prescription group were （25.4 ±4.30） times, （25.2±4.02） times, （24.1±5.51 ） times respec- tively, which were significantly higher than that of the model group （P 〈 0.05 ）. That of Naofukang group and Jianpi Yishen Prescription group after treatment was （20.0 ± 2.31 ） times, （ 17.0± 3.16） times and （23.5 ±4.71 ） times respectively,which were significantly lower than that of the model group （P 〈 0.05 ）. That of Jianpi Yishen Prescription group significantly lower than that of the Naofukang group（P 〈 0.05）. The expression of JNK protein in model group, Naofukang group and Jianpi Yishenfang group were （32.9 ± 4.82）, （28.6± 5.32）, （22.4±4.90） respectively and that of the Naofukang group and Jianpi Yishen Prescription group was significantly lower than that of the model group （ P 〈 0.05 ）. That of the Jianpi Yishen Prescription group was significantly lower than that of the Naofukang group （P 〈 0.05 ）. Conclusion ：Jianpi Yishen Prescription can improve the learning and memory ability of AD rats and the mechanism may be related to the decrease of JNK protein expression in hippocampal CA1 region.

关 键 词：健脾益肾方 老年性痴呆 记忆能力 C-JUN氨基末端激酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]