利用植物悬浮细胞体系对骆驼蓬碱进行生物转化  

Biotransformation of Harmaline by Plant Cell Suspension Cultures

作　　者：赵爽[1,2] 刘颖[1] 王加利[1] 袁将 姬瑞芳 全庆华 王贝贝[1] 董洁[1] 冀娇娇 刘永刚[1] 

机构地区：[1]北京中医药大学,北京100102 [2]清河县疾病预防控制中心,河北清河054800

出　　处：《中医学报》2017年第1期103-105,123,共4页Acta Chinese Medicine

基　　金：新疆教育厅项目基金(XJEDU2012S43)

摘　　要：目的:利用植物悬浮细胞培养体系对骆驼蓬碱进行生物转化。方法:利用长春花、一叶萩、烟草和金银花植物悬浮细胞培养体系转化骆驼蓬碱,应用薄层色谱法和质谱法对转化结果进行检识。结果:4种植物悬浮细胞培养体系中均检测到新的转化产物,该转化产物采用碘化铋钾溶液在薄层色谱上显色,在正离子模式下,准分子离子为213.0,二级碎片为198.1、170.2,初步推测为去氢骆驼蓬碱。结论:利用植物悬浮细胞培养体系生物转化的方法可以将骆驼蓬碱转化成去氢骆驼蓬碱。Objective ： To study the biotransformation of harmaline by plant cell suspension cultures. Methods ： Cell suspension cul- tures of Catharanthusroseus, Securinegasuffruticosa, Nicotianatabacum and Lonicera japonica were used to realize the biotransfor- mation of harmaline. The results were examined with TLC and MS. Results ： One biotransformation product was found in the four plant cell suspension cultures. TLC results showed that the product was colored by bismuth potassium iodide solution, and the MS results showed that the quasi-molecular ion peak was 213.0 and the two fragment peaks in the second-order mass spectrum were 198.1 and 170.2, which suggested that the biotransformation product may be harmine. Conclusion：Harmaline can be converted in- to harmine by plant cell suspension cultures.

关 键 词：植物悬浮细胞体系 骆驼蓬碱 去氢骆驼蓬碱 生物转化 中药 

分 类 号：R284.2[医药卫生—中药学]