伊洛前列素对低氧性肺动脉高压大鼠肺部内皮素-1表达的影响  

Effects of iloprost on expression of Endothelin-1 in lung in rats with hypoxic pulmonary hypertension

作　　者：官莉[1] 金柱[1] 陈世雄[1] 向光明[1] 高宝安[1] 

机构地区：[1]三峡大学呼吸病研究所/湖北省宜昌市中心人民医院呼吸内科,湖北宜昌443003

出　　处：《重庆医学》2017年第6期735-737,741,共4页Chongqing medicine

基　　金：湖北省自然科学基金资助项目(2012FFB06301);湖北省自然科学基金指导性计划项目(2014CFC1037)

摘　　要：目的探讨伊洛前列素对低氧性肺动脉高压(HPH)大鼠肺部内皮素-1(ET-1)的影响。方法雄性SD大鼠32只,分为正常(NC)组,正常治疗(NT)组,低氧(HC))组,低氧治疗(HT)组,每组8只。NC组和NT组在常氧条件下饲养3周;HC和HT组放置于低氧舱(O2浓度10%)进行缺氧处理3周(8h/d);NT和HT组每天进行伊洛前列素雾化吸入治疗(2μg/kg)。第3周结束时,测量各组平均肺动脉压(mPAP),右心室收缩期末压力(RVSP),右心室肥厚指数。HE染色观察各组肺动脉形态学变化,计算肺小动脉的血管壁厚百分比(WT%),RT-PCR分析各组肺组织匀浆中ET-1的变化趋势。结果 HC组大鼠血流动力学指标较其他组明显升高,HT组相关指标表达与NC组比较差异无统计学意义(P>0.05)。HC组较其他组肺动脉管壁增厚、管腔狭窄明显;HT组则有不同程度逆转。HC组大鼠肺组织匀浆中ET-1表达较NC组明显升高,HT组中ET-1表达与NC组比较差异无统计学意义(P>0.05)。结论伊洛前列素对HPH有明显的治疗作用,可以降低HPH大鼠肺部ET-1的释放,阻止肺血管重塑。Objective To investigate the influence of iloprost on the expression of lung endothelin-1（ET-1）in rats with hypoxic pulmonary hypertension（HPH）.Methods Thirty-two male SD rats were randomly divided into four groups：normal control（NC）group,normal treatment（NT）group,hypoxia control（HC）group,hypoxia treatment（HT）group.NC group and NT group raised under normal Oxygen conditions 3 weeks.HC and HT group placed in a low Oxygen chamber（O2 10%）were treated 3weeks of hypoxia 8hours per day.NT and HT group were treated daily iloprost by inhalation therapy（2μg/kg）.Affer three weeks,measured mean pulmonary arterial pressure（mPAP）,right ventricular systolic pressure（RVSP）,index of right ventricular hypertrophy.HE staining was used to observe the morphological changes of pulmonary arteries and image analysis system was used to calculate the percentage of vascular wall thickness of small pulmonary arteries in each group,RT-PCR technique was used to assess the trends of ET-1in lung tissue homogenates.Results The hemodynamics in HC group was significantly higher than other groups（P〈0.05）,there was no significant statistically difference of the hemodynamics in HT group compare with normal control group.Pulmonary arteries morphology,vessel wall thickening and vessel lumina stenosis in HC group than NC、NT、group.These indicators were significant improved in HT group.ET-1expression in lung tissue were significantly increased in HC group than NC groups.No significant difference was found between and NC group HT group.Conclusion Aerosol inhalation of iloprost has exact therapeutic effect for rats with HPH.Iloprost reduced ET-1overexpression in lung tissue in HPH rats and prevent pulmonary vascular remodeling.

关 键 词：高血压 肺性 缺氧 伊洛前列素 内皮素-1 

分 类 号：R544.1[医药卫生—心血管疾病]