辣椒轻斑驳花叶病毒寿光分离物的RT-PCR检测及其序列分析  

Identification and Sequence Analysis of Shouguang Isolate by RT-PCR of Pepper Mild Mottle Mosaic Virus

作　　者：唐玉海[1] 乔宁[2] 孙晓辉[2] 

机构地区：[1]潍坊科技学院贾思勰农学院,山东寿光262700 [2]山东农业大学植保学院,山东省蔬菜病虫生物学重点实验室,山东泰安271018

出　　处：《北方园艺》2017年第4期94-97,共4页Northern Horticulture

基　　金：公益性行业(农业)科研专项资助项目(201303028);山东省自然科学基金资助项目(ZR2012CM032);山东省科技发展计划资助项目(2010GNC10915)

摘　　要：以山东寿光地区感染辣椒轻斑驳花叶病毒的辣椒为试材,采用试剂盒提取感病辣椒的总RNA并进一步反转录成cDNA,参照已报道的PMMoV检测引物合成特异性引物PMMoV-F和PMMoV-R;以获得的cDNA为模板进行PCR扩增,研究了辣椒轻斑驳花叶病毒寿光分离物。结果表明:得到分子量约是576bp的条带,纯化后进行基因测序;通过测序结果分析比对可知,PMMoV寿光分离物序列与诸多地区的分离物同源性较高,可达到99%~100%。Peppers infected by PMMoV in Shouguang city,Shandong Province were used as test material and kit was used to extract total RNA from infected pepper and total RNA was further reversed into cDNA by reverse transcription.PMMoV-F and PMMoV-R was synthesized according to reported specific primers.The cDNA of PMMoV isolate was used for PCR amplication.The results showed that the molecular weight of band was576 bp.Sequence analysis showed that PMMoV infected pepper in Shouguang had closer genetic relationship with PMMoV isolated in other areas,which could achieve 99%-100%.

关 键 词：辣椒轻斑驳花叶病毒 寿光分离物 RT-PCR 序列分析 

分 类 号：S641.3[农业科学—蔬菜学]