走马胎离体培养及植株再生  

In vitro Culture and Plant Regeneration of Ardisia gigantifolia Stapf.

作　　者：符运柳[1,2] 徐立[1,2] 李志英 黄碧兰[1,2] 李克烈[1,2] 

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [2]农业部华南作物基因资源与种质创制重点实验室,海南省热带作物种质资源遗传改良与创新重点实验室,海南儋州571737

出　　处：《北方园艺》2017年第4期98-101,共4页Northern Horticulture

基　　金：农业部引进国际先进技术资助项目(2015-Z22);农业部物种资源保护专项资助项目(16RZZY-101)

摘　　要：以走马胎幼嫩叶片为外植体,研究不同培养基对走马胎叶片愈伤组织诱导、不定芽分化、增殖及生根培养的影响。结果表明:叶片在MS+6-BA 1.0mg·L^(-1)+NAA 1.0mg·L^(-1)培养基上诱导产生的愈伤组织最适合用于进行分化和增殖;愈伤组织分化不定芽的最适培养基为MS+6-BA 2.0mg·L^(-1)+NAA 0.1mg·L^(-1),分化率高达88.9%;壮苗培养基为MS+6-BA0.5mg·L^(-1)+NAA 0.1mg·L^(-1)+10%椰子水(CW);最适的生根培养基为MS+NAA0.1mg·L^(-1)+IBA 1.0mg·L^(-1),生根率达100%,根系发达,植株生长健壮。经生根培养及练苗,走马胎的假植成活率达85%。A.gigantifolia Stapf.leaves were used as explants,the effects of different mediums on leaf callus induction,adventitious bud differentiation,proliferation and rooting were studied.The results showed that the callus induced from leaf explants on medium MS＋6-BA 1.0mg·L^-1＋NAA 1.0mg·L^-1 were easy to produce auxiliary shoots.The differentiation rate was 88.9%.The suitable medium for bud subculture and proliferation was MS＋6-BA 2.0mg·L^-1＋NAA 0.1mg·L^-1.The elongation medium was MS＋6-BA 0.5mg·L^-1＋NAA 0.1mg·L^-1＋10% coconut water.The optimal rooting medium was MS＋NAA 0.1mg·L^-1＋IBA1.0mg·L^-1 with rooting rate as high as 100%.The roots developed well and the plantlets grew vigorously.The plantlets with well-developed roots were successfully acclimatized and planted with a survival rate of 85%.

关 键 词：走马胎 叶片 离体培养 植株再生 

分 类 号：S793.9[农业科学—林木遗传育种]