斑蝥酸镁抑制人胃癌细胞BGC-823增殖及其机制  

In vitro research of proliferative inhibition of human gastric cancer cell BGC - 823 by magnesium cantharidate and the underlying mechanism

作　　者：梁金龙[1] 谢铭[1] 杨雪峰[1] 

机构地区：[1]遵义医学院附属医院胃肠外科,贵州遵义563003

出　　处：《中华实验外科杂志》2017年第2期195-198,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨斑蝥酸镁在体外对人胃癌细胞BGC-823增殖的抑制作用及其机制。 方法分别加入0.52、1.04、2.08、4.17、8.34 μmol/L的斑蝥酸镁作用24 h后，细胞计数试剂盒（CCK-8）法检测BGC-823细胞增殖的影响；光学显微镜和透射电镜观察细胞形态学和细胞超微结构的变化；碘化丙锭（PI）单染色法检测细胞周期变化，膜联蛋白V-异硫氰酸荧光素（Annexin V-FITC）/PI双染流式细胞术检测细胞凋亡水平；Western blot检测细胞周期蛋白依赖性激酶1（CDK1）、细胞周期蛋白（Cyclin） B1蛋白的表达。 结果不同浓度斑蝥酸镁作用24 h后，BGC-823细胞均出现抑制现象，且呈剂量-效应关系（P＝0.000），其半数抑制浓度（IC50）为4.868 μmol/L；IC50的斑蝥酸镁作用24 h后，BGC-823的细胞核异形、裂解，内质网及线粒体肿胀；加入4.868 μmol/L斑蝥酸镁作用24 h后，BGC-823细胞的凋亡率明显增加（P＝0.010），且表现出明显的G2/M期阻滞（P＝0.009）；加入2.434、4.868、7.302 μmol/L斑蝥酸镁作用24 h后，BGC-823细胞的CDK1、Cyclin B1表达明显下降（P＝0.000）。 结论斑蝥酸镁能够抑制胃癌细胞BGC-823增殖，并将细胞阻滞在G2/M期。Objective To evaluate the inhibitory effects of magnesium cantharidate on human gas- tric cancer cell BGC - 823 lines in vitro and the relative mechanisms. Methods Twenty - four h after adding 0. 52, 1.04, 2. 08, 4. 17, and 8. 34 μmol/L magnesium cantharidate, cell counting kit -8 （ CCK - 8 ） assay was used to observe the proliferation of BGC - 823 cells ; morphological changes of apop- tosis were observed using the light microscope and trans mission electron microscope; propidiumiodide （PI） single staining was used to detect the changes of cell cycle, and apoptotic cell percentage was detec- ted by flow cytometry; The expression levels of cyclin -dependent kinase 1 （CDK1） and cyclin B1 pro- teins were tested by Western blotting. Results Magnesium cantharidate had obviously an inhibitory effect on BGC - 823 ceils in a dose - dependent manner （ P = 0. 000）. The inhibitory concentration （ IC5 ） of magnesium cantharidate was 4. 868μmol/L. 24 h after treatment with 4. 868μmol/L magnesium canthari- date, BGC- 823 cells represented nuclear abnormity, cleavage and fragmentation, and endoplasmic reticulum and mitoehondria swelling. Affter treatment with 4. 868 μmol/L magnesium cantharidate for 24 h, the apoptosis rate of BGC -823 cells increased （P = 0. 010） and the cells were significantly blocked in G2/M phase （ P = 0. 009）. 24 h after treatment with 2. 434, 4. 868, 7. 302 μmol/L magnesium cantharidate, the expression of CDK1 and Cyclin B1 decreased significantly in BGC -823 cells （P = 0. 000）. Conclusion Magnesium eantharidate can inhibit the proliferation of BGC - 823 cells, and significantly block the cells in G2/M phase.

关 键 词：斑蝥酸镁 胃癌 半数抑制浓度 细胞周期 周期蛋白 

分 类 号：R285[医药卫生—中药学]