热量限制对老年小鼠骨骼肌卫星细胞增殖能力的影响  

The effect of calorie restriction on the proliferation of satellite cells in elderly rats＇ skeletal muscles

作　　者：白川川 蒲荣喜[2] 舒彬[1] 杨忠[2] 张凌云[1] 

机构地区：[1]重庆医科大学附属大学城医院康复中心,重庆401331 [2]第三军医大学西南医院临床血液学教研室

出　　处：《中华物理医学与康复杂志》2017年第2期92-96,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金资助项目（31571242）

摘　　要：目的探讨热量限制对老龄小鼠骨骼肌卫星细胞增殖能力减退的延缓作用。方法选取12～13月龄的C57BL雄性小鼠12只，按随机数字表法均分为实验组和对照组，每组6只，均单笼饲养。实验开始前，2组小鼠均适应性饲养2周，计算平均进食量作为对照组投食量，约75．09kJ／d；实验组采用热量限制法投食，投食量为对照组的60％，约45．05kJ／d。逐日投放饲料，每周称量体重一次，至实验第15周结束。分别比较2组小鼠实验前和实验第15周（实验后）的体重变化。实验第15周后分别取后肢骨骼肌制备单核细胞悬液，采用流式细胞术分选小鼠肌卫星细胞，并进行细胞周期测定；用免疫印迹法检测细胞周期蛋白（cyclin）A、cyclinD1、cyclinE的表达，并进行统计学分析比较。结果实验前，实验组和对照组小鼠体重分别为（30．7±0．4）g和（30．8±0．3）g，组间差异无统计学意义（P〉0．05）；实验后，实验组小鼠的体重降至（19．5±0．4）g，较组内实验前明显降低（P〈0．001），且明显低于对照组小鼠的体重[（31．9±0．5）g]，组间差异有统计学意义（P〈0．001）。与对照组[G0／G1期（89．78±0．37）％；S期（4．29±1．57）％]相比，实验组骨骼肌卫星细胞的G。／G，期比例[（62．18±0．42）％]明显下降（P〈0．001），S期比例[（28．75±0．30）％]显著升高（P〈0．001）；而且cyclinA（P〈0．001）、cyclinE（P〈0．01）表达增强，cyclinD1表达降低（P〈0．01）。结论热量限制可以延缓老龄小鼠骨骼肌卫星细胞增殖能力的减退。Objective To explore any effect of calorie restriction on the proliferation of satellite cells in the skeletal muscles of elderly rats. Methods Twelve male C57BL rats aged 12 or 13 months were randomly divided into an experimental group and a control group, each of 6. The control group was fed 75.09 kJ/d as normal, while the experimental group was provided with 45.05 kJ/d （60% of normal）. The intervention lasted for 15 weeks and each rat＇s weight was measured every week. After the intervention, limb muscle satellite cells were sorted by fluorescenceactivated cell sorting after digestion, and the cell cycle was analyzed. Western blotting was used to assess the expression of cyclin A, D1 and E. Results There was no significant difference in the average weight of the two groups before the experiment. After the 15 weeks the average weight of the experimental group had decreased significantly （ to 19.5±0.4 g） , and it was significantly lighter than that of the control group （ 31.9±0.5 g）. The average percentage of the satellite cells in the G0/G1 phase had decreased significantly in the experimental group, but the percentage in the S phase had increased significantly. The expression of cyclin A and E was significantly greater in the experimental group compared with the control group, but the expression of cyclin D1 had decreased significantly. Conclusion Caloric restriction can delay the proliferation of satellite cells in the skeletal muscles of elderly mice.

关 键 词：热量限制 衰老 肌卫星细胞 增殖 

分 类 号：R339.38[医药卫生—人体生理学]