Pb^(2+)与BSA的结合平衡研究  

STUDY ON THE BINDING EQUILIBRIUM BETWEEN Pb^(2+) WITH BSA

作　　者：程艳坤[1] 闫志谦[1] 王立璇[1] 康爱彬[1] 

机构地区：[1]河北化工医药职业技术学院,石家庄050026

出　　处：《离子交换与吸附》2017年第1期65-71,共7页Ion Exchange and Adsorption

基　　金：河北省自然科学基金资助项目(B2006000308)

摘　　要：在pH 6.3条件下,利用平衡透析法和紫外吸收光谱法研究了Pb^(2+)与BSA的相互作用。用紫外光谱法研究了Pb^(2+)与BSA相互作用的波长为278nm;由Scatchard图得到Pb^(2+)在BSA上的结合位点数为1.6个强结合部位;吸光度随时间的变化反应确定为一级反应;用非线性最小二乘法拟合Bjerrum公式求得逐级稳定常数。The binding of Pb2＋ to BSA has been studied by equilibrium dialysis and ultra-visible absorption spectrum at pH=6.3, Wavelength about the binding of Pb2＋in BSA by Ultraviolet Visible Spectrophotometer was 278nm. The Scatchard analysis indicated that there existed 1.6 strong bindings site of Pb2＋ in BSA. Reaction of absorbance change over time was a first order reaction, Pb2＋-BSA system was determined by non-linear least-square method fitting Bjerrun formula.

关 键 词：PB2+ BSA 紫外光谱法 平衡透析法 逐级稳定常数 

分 类 号：O657.3[理学—分析化学]