ITS2条形码对白及属植物的初步分析研究  

Basic Research on Bletilla Based on ITS2 Barcode

作　　者：宋爽[1] 周洋帆[1] 黄丽[1] 杨娟[1] 文国松[1] 徐绍忠[1] 查应洪 赵明富[1] 赵昶灵[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]红河群鑫石斛种植有限公司,云南红河661200

出　　处：《云南农业大学学报（自然科学版）》2017年第1期95-100,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：农业科技成果转化资金(2013GB2F300441)

摘　　要：本研究通过ITS2条形码对云南白及本地种的分布情况初步了解,并对药用白及和云南本地种进行分析研究。选取滇东南、滇东北、滇西北、滇西的材料,进行DNA提取、PCR、测序,MEGA 5.1软件进行序列比对、NJ系统聚类树构建,并预测ITS2二级结构。结果表明:药用白及和本地种ITS2序列之间存在明显差异,在NJ树上分别聚为两类。ITS2可以区分药用白及和本地种,且可以作为初步分析药用白及真伪的潜在条形码。In this study, we used the ITS2 barcode to identify the distribution of indigenous species of Bletilla striata in Yunnan, analyzed the officinal species and indigenous species. Materials were gathered from the southeast, northeast, northwest and western region of Yunnan. Then the genomic DNA was isolated, ITS2 sequence was amplified and sequenced. Analytic identifications were per- formed by MEGA 5.1, then predicted the secondary structure of ITS2 sequences. The results showed that a significant difference was observed between the ITS2 sequence of B. striata and indige- nous species, they were singly divided into two classes in the NJ tree. The study showed that the ITS2 barcode could differentiate medicinal material and indigenous species of B. striata, and could be used as a potential DNA barcode to preliminarily analyze the medicinal plants B. striata and its a- dulterants.

关 键 词：白及 ITS2 物种鉴定 

分 类 号：R282.5[医药卫生—中药学] S567.239[医药卫生—中医学]