高效液相色谱法测定磺胺间甲氧嘧啶的含量  

Determination of Sulfamonomethoxine by HPLC

作　　者：陈锡龙[1] 

机构地区：[1]贵州省兽药饲料监察所,贵阳550003

出　　处：《中国兽药杂志》2017年第2期16-19,共4页Chinese Journal of Veterinary Drug

摘　　要：建立了兽药磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶含量的高效液相色谱测定法。以C18为固定相,0.1%磷酸溶液-乙腈(80∶20)为流动相,流速1.0 m L/min,柱温40℃,检测波长270 nm,采用外标法进行定量。磺胺间甲氧嘧啶的线性范围为5.0~100.0μg/m L,回归方程为Area=40.333×Amt-18.843(n=5),相关系数大于0.999。从高(120%)、中(100%)和低(80%)三个添加水平的检测结果可以看出,磺胺间甲氧嘧啶的回收率为96.27%~98.30%。该方法简单,准确,精密度好,回收率高,可以用于测量磺胺间甲氧嘧啶钠注射液中磺胺间甲氧嘧啶的含量。An HPLC method has been developed to determine the content of sulfamonomethoxine in sulfamonomethoxine sodium injection, using C18 column as stationary phase, with a mobile phase of 0. 1% phosphoric solution - acetonitrile （ 80 ： 20 ） and wavelength of 270 nm. It was quantified by external standard method and the linear range of sulfamonomethoxine was between 5.0 and 100.0 p,g/mL. And the regression equation was Area =40.333 xAmt- 18. 843 （n = 5） with the correlation to be more than 0. 999. The average recoveries at three different levels of 120%, 100% and 80% of the sample ranged between 96. 27% and 98.30%. This method was simple and accurate, and had a good precision and high recoveries as well, could be used for determination of sulfamonomethoxine in sulfamonomethoxine sodium injection

关 键 词：磺胺间甲氧嘧啶 含量 高效液相色谱法 

分 类 号：S859.2[农业科学—临床兽医学]