Carba NP试验检测碳青霉烯类耐药肠杆菌科细菌的临床应用  

Clinical application of Carba NP test in detecting CRE strains

作　　者：李渊婷[1] 金凤玲[1] 

机构地区：[1]兰州大学第一医院检验科,甘肃兰州730000

出　　处：《西安交通大学学报（医学版）》2017年第2期304-309,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的探讨Carba NP试验检测碳青霉烯类耐药肠杆菌科细菌的临床应用。方法收集碳青霉烯类抗生素耐药的51株肠杆菌科细菌,K-B法药敏试验检测常规药物敏感性,E-test法检测试验菌株对碳青霉烯类抗生素的最低抑菌浓度(MIC)值;分别用改良Hodge、Carba NPⅠ试验筛选碳青霉烯酶表型;然后用Carba NPⅡ试验进行碳青霉烯酶表型分类,E-test法测定金属酶,用PCR检测耐药基因。结果 41株Carba NPⅠ试验阳性且经Carba NPⅡ试验证实为产B类金属酶菌株,PCR均证实携带NDM-1基因型。结论 Carba NP试验方便快捷、结果易于判断,与耐药基因检测符合程度高,适合实验室常规开展。Objective To investigate the clinical application of Carba NP test in detecting carbapenemresistant enterobacteriaceae.Methods Routine method was adopted to identify the 51 strains from clinical isolates and K-B test was conducted to screen out the carbapenem-resistant enterobacteriaceae and detect antimicrobial susceptibility.E-test was used to detect the minimum inhibitory concentration（MIC）of carbapenems.Modified Hodge test（MHT）and Carba NPⅠ test were used to screen out the phenotypes of carbapenemase,respectively.Then Carba NPⅡtest was used to classify carbapenemase which were positive in Carba NP I test.E-test was used for the detection of metalloenzymes.Polymerase chain reaction（PCR）was used for the detection of resistance genes.Results Totally 41 strains were positive in Carba NP1 test and classified as B-class by Carba NPⅡtest.They were positive in metalloenzyme by E-test and carried NDM-1 gene by PCR.Conclusion Carba NP test can be used conveniently,has high consistency with PCR method,and the result is easy to determine.Therefore,it can be used as a routine laboratory method.

关 键 词：碳青霉烯酶 CARBA NP试验 肠杆菌科 耐药基因 

分 类 号：R446.5[医药卫生—诊断学]