人参皂苷Rb1减少间歇性高糖诱导雪旺细胞氧化应激  

Effect of Ginsenoside Rb1 on Oxidative Stress in Schwann Cells Induced by Intermittent High Glucose

作　　者：薛冰[1] 梁琳琅[1] 张颖[1] 孙连庆[2] 

机构地区：[1]沈阳军区总医院,辽宁沈阳110016 [2]西安交通大学第一附属医院,陕西西安710061

出　　处：《辽宁中医药大学学报》2017年第2期10-12,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81673785);陕西省自然科学基础研究计划重点项目(2015JZ023);陕西省自然科学基金项目(2014JM4118);陕西省中医药管理局项目(13-LC088)

摘　　要：目的:探讨人参皂苷Rb1对间歇性高糖培养条件下雪旺细胞氧化应激的影响。方法:无菌剥取新生Sprague-Dawley乳鼠坐骨神经,体外制备雪旺细胞,分为对照组(培养液葡萄糖浓度为5.6 mmol/L,Con组)、间歇性高糖组(培养液葡萄糖浓度为5.6 mmol/L和50 mmol/L每8 h交替,IHG组)、干预组(在间歇性高糖基础上加入100μmol/L人参皂苷Rb1,IHG+Rb1组)分别培养48 h。MTT法检测细胞活性,流式细胞仪检测细胞内ROS,提取DNA后采用ELISA法检测8-OHd G。结果:IHG组雪旺细胞活性明显下降,是Con组的70.1%(P<0.05);ROS、8-OHd G水平均明显高于Con组,分别是Con组的2.86倍、3.24倍(P<0.01)。人参皂苷Rb1干预可以改善间歇性高糖导致的细胞活性下降,IHG+Rb1组细胞活性增加到IHG组的1.27倍(P<0.05);人参皂苷Rb1抑制了间歇性高糖引起的ROS、8-OHd G升高,IHG+Rb1组ROS、8-OHd G水平分别较IHG组下降了36.7%和32.7%(P<0.05)。结论:间歇性高糖可诱导雪旺细胞产生氧化应激造成神经细胞损伤,人参皂苷Rb1能够减轻上述反应,具有一定的神经保护作用。Objective： To investigate the protective effects of ginsenoside Rbl on oxidative stress in Schwann cells induced by intermittent high glucose （ IHG ）. Methods ： Schwann cells were obtained from the sciatic nerves of neonatal Sprague-Dawley rats and primarily cultured. The cultured cells were treated for 48 h with 5.6 mmol/L glucose as control （ Con group ）, or with 5.6 mmol/L and 50 mmol/L glucose alternating every 8 h to mimic IHG （ IHG group ）, or with IHG plus100 μ mol/L ginsenoside Rbl （ IHG＋Rbl group ）. Cell viability was detected by MTT assay. Intracellular reactive oxygen species （ ROS ） production was measured by flow cytometry. 8-hydroxy-2-deoxy guanosine （ 8-OHdG ） level was measured by enzyme linked immunosorbent assay. Results：The cell viability in IHG group was decreased to 70.1% of that in Con group （P〈0.05）. The levels of ROS and 8-OHdG in IHG group （ 2.86 and 3.24 folds of control respectively ）were significantly increased as compared with Con group （ P〈0.01 ）. 100 μ mol/L Rbl treatment attenuated the above effects induced by IHG, the cell viability in IHG＋Rbl group was 1.27 fold of that in IHG group, the levels of ROS and 8-OHdG in IHG＋Rbl group were decreased by 36.7% and 32.6% respectively as compared with IHG group （P〈0.05）. Conclusion： Ginsenoside Rbl could attenuate IHG induced oxidative stress in Schwann cells, thereby might be useful in DPN treatment.

关 键 词：人参皂苷RB1 雪旺细胞 氧化应激 

分 类 号：R587.2[医药卫生—内分泌]