他扎罗汀对角质形成细胞增殖周期蛋白Cyclin D1/CDK4/CDK6表达水平的影响  

Effects of tazarotene on the expression of Cyclin D1/CDK4/CDK6 in human epidermal keratinocytes

作　　者：邢立亚 高丽琴[1] 贾静[1] 李霞[1] 

机构地区：[1]西安市第一医院皮肤科,陕西西安710002

出　　处：《临床皮肤科杂志》2017年第3期165-168,共4页Journal of Clinical Dermatology

基　　金：西安市科技计划基金(SF1204)资助项目

摘　　要：目的:探讨他扎罗汀(Tazarotene)对角质形成细胞增殖周期蛋白Cyclin D1、CDK4和CDK6表达水平的影响。方法:分别以不同浓度的他扎罗汀作用于HaCaT细胞,用CCK-8比色法检测每组细胞在12、24、48和72 h的增殖情况,流式细胞仪检测细胞周期变化,western blot检测周期相关蛋白水平变化。结果:他扎罗汀对HaCaT细胞具有明显的增殖抑制作用(F=21.23,P<0.05),并使G0/G1期细胞比例增加(F=19.83,P<0.05),周期相关蛋白Cyclin D1、CDK4、CDK6的表达水平明显下调。结论:他扎罗汀对HaCaT细胞具有明显的增殖抑制和周期阻滞作用,其作用机制可能与下调Cyclin D1/CDK4/CDK6蛋白复合物的表达有关。Objective： To investigate the effects of tazarotene on the expression of Cyclin D1/CDK4/CDK6 in keratinocytes. Methods： Keratinocytes（HaCaT） were treated with different concentrations of tazarotene. Cell proliferation was determined with CCK-8 assay after 12, 24, 48 and 72h of treatment. Changes in cell cycle and cycle related protein levels were assessed with flow cytometry and western blot, respectively. Results： Tazarotene dramatically inhibited proliferation of HaCaT cells in a dose-de- pendent manner（F=21.23, P〈0.05）, and increased the proportion of cells in G0/G1 phase（F=19.83, P〈0.05）. Tazarotene signifi- cantly decreased expression levels of Cyclin D1, CDK4 and CDK6 proteins. Conclusion： Tazarotene inhibits keratinocyte prolif- eration and cell cycle progression, possibly via down regulation of expression levels of Cyclin D1/CDK4/CDK6 protein complex.

关 键 词：他扎罗汀 人角质形成细胞 细胞增殖 细胞周期 周期素 周期素依赖性激酶 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]