急性腹膜炎小鼠心肌炎性介质产生及组织学变化  

Secretion and histological changes of inflammatory mediators in myocardium of acute peritonitis mice

作　　者：税利蓉 

机构地区：[1]西南医科大学附属中医院,四川泸州646000

出　　处：《临床医学研究与实践》2017年第4期4-5,8,共3页Clinical Research and Practice

摘　　要：目的研究大肠杆菌感染导致急性腹膜炎发生后小鼠血循环及心肌内重要炎性介质(IL-1β和TNF-α)的产生和变化情况,以及心肌组织的形态学变化。方法随机将小鼠分为正常对照组、急性细菌性腹膜炎组(小鼠腹腔内注射3×10~8 cfu/mL浓度的大肠杆菌菌液引发细菌性腹膜炎),采用放射免疫分析法检测不同时间点小鼠心肌内TNF-α和IL-1β含量,再通过病理形态学技术,观察心肌组织的形态学改变。结果正常小鼠血循环及心肌内含有一定量IL-1β和TNF-α,与正常对照组相比,急性细菌性腹膜炎组小鼠血循环及心肌内的IL-1β和TNF-α均有不同程度升高(P<0.05)。病理形态学观察表明,急性细菌性腹膜炎发生后,炎症介质增高使小鼠心肌组织表现出炎症改变,主要为充血、出血、渗出及炎性细胞浸润,以感染后24 h更为明显。结论急性细菌性腹膜炎可致血循环及心肌内IL-1β和TNF-α过度分泌,并使心肌出现炎症反应,即急性细菌性腹膜炎可诱导心肌炎症的发生。Objective To study the secretion and changes of the important inflammatory mediators（IL-1β and TNF-α）in the blood circulation and myocardium in mice after acute peritonitis caused by Escherichia coli infection, as well as myocardial histological changes. Methods The mice were randomly divided into normal control group and acute bacterial peritonitis group（mice were intraperitoneally injected with 3 ×10-8cfu/m L Escherichia coli bacteria to induce bacterial peritonitis）. The contents of TNF-α and IL-1β in myocardium at different time points of mice were detected by radioimmunoassay, then the morphological changes of myocardium were observed by pathomorphology. Results Normal mice blood circulation and myocardium contain a certain amount of IL-1β and TNF-α, the levels of IL-1β and TNF-αin the blood of the mice with acute bacterial peritonitis group were higher than those in the control group（P〈0.05）.Histopathological observation showed that the inflammatory mediators increased after acute bacterial peritonitis, and the myocardial tissue showed inflammatory changes, mainly for congestion, bleeding, exudation and inflammatory cell infiltration, and they was more obvious 24 h after infection. Conclusion Acute bacterial peritonitis can cause over-secretion of IL-1β and TNF-α in blood circulation and myocardial and myocardial inflammation, in other words, acute bacterial peritonitis can induce the occurrence of myocardial inflammation.

关 键 词：急性细菌性腹膜炎 炎症介质 肿瘤坏死因子-α 白细胞介素-1Β 

分 类 号：R259[医药卫生—中西医结合]