Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus andtheir associationwith Vibrio alginolyticus  

作　　者：刘盟 刘媛 惠敏 宋呈文 崔朝霞 

机构地区：[1]College of Fisheries andLife Science, Dalian Ocean University, Dalian 116023, China [2]Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [3]Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China [4]National & Local Joint Engineering Laboratory of Ecological Marieulture, Qingdao 266071, China

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第2期235-243,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.41206147,31302187);the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02)

摘　　要：Clip domain serine proteases （cSPs） and their homologs （SPHs） play an important role in various biological processes that are essential components of extracellular signaling cascades, especially in the innate immune responses of invertebrates. Here, polymorphisms of PtcSP and PtSPH from the swimming crab Portunus tritubereulatus were investigated to explore their association with resistance/ susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X, and characterized with SPSS 16.0 software, and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a Zz test. A total of 109 and 77 single nucleotide polymorphisms （SNPs） were identified in the genomic fragments of PtcSP and PtSPH, respectively. Notably, nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to I1. alginolyticus （P〈0.05）. Among them, eight SNPs were observed in introns, and one synonymous, four non-synonymous SNPs and one ins-del were found in coding exons. In addition, five simple sequence repeats （SSRs） were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies, the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After fiarther validation, polymorphisms investigated here might be applied to select potential molecular markers ofP. trituberculatus with resistance to I1. alginolyticus.Clip domain serine proteases(cSPs) and their homologs(SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades,especially in the innate immune responses of invertebrates.Here,polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus.Polymorphic loci were identified using Clustal X,and characterized with SPSS 16.0 software,and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a χ~2 test.A total of 109 and 77 single nucleotide polymorphisms(SNPs) were identified in the genomic fragments of PtcSP and PtSPH,respectively.Notably,nearly half of PtSPH polymorphisms were found in the non-coding exon 1.Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V.alginolyticus(P<0.05).Among them,eight SNPs were observed in introns,and one synonymous,four non-synonymous SNPs and one ins-del were found in coding exons.In addition,five simple sequence repeats(SSRs) were detected in intron 3 of PtcSP.Although there was no statistically significant difference of allele frequencies,the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks.After further validation,polymorphisms investigated here might be applied to select potential molecular markers of P.trituberculatus with resistance to V.alginolyticus.

关 键 词：Portunus trituberculatus clip domain serine proteinase serine proteinase homolog POLYMORPHISM susceptibility/resistance 

分 类 号：S917.4[农业科学—水产科学]