Biomass and nutrient productivities ofTetraselmis chuii under mixotrophic culture conditions with various C：N ratios  

作　　者：鹿琳 王军 杨官品 朱葆华 潘克厚 

机构地区：[1]Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China [2]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China [3]Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

出　　处：《Chinese Journal of Oceanology and Limnology》2017年第2期303-312,共10页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China(973Program)(Nos.2011CB200901,2011CB200904);the National Key Technology Research and Development Program(No.2011BAD14B01);the National High Technology Research and Development Program of China(863 Program)(No.2013AA065801);the Special Foundation for Marine Renewable Energy(No.GHME2011SW03)

摘　　要：Mass microalgal culture plays an irreplaceable role in aquaculture,but microalgal productivity is restricted by traditional autotrophic culture conditions.In the present study,a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea.The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon:nitrogen(C:N)ratios than those under autotrophic conditions.When the C:N ratio was 16,the optical density and biomass productivity were 3.7-and 5-fold higher than their corresponding values under autotrophic culture conditions,respectively.Moreover,T.chuii synthesized more polysaccharides and total lipids under mixotrophic conditions.In addition,T.chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions.At a C:N ratio of 16,the percentage of C16:0 and C18:1 reached 30.08%and 24.65%of the total fatty acid(TFA) content,respectively.These findings may provide a basis for largescale mixotrophic culture of T.chuii,as a potential bait-microalga.Mass microalgal culture plays an irreplaceable role in aquaculture, but microalgal productivity is restricted by traditional autotrophic culture conditions. In the present study, a Tetraselmis chuii strain belonging to the phylum Chlorophyta was isolated from south Yellow Sea. The growth rate and biomass productivity of this strain was higher under mixotrophic conditions with different carbon：nitrogen （C：N） ratios than those under autotrophic conditions. When the C：N ratio was 16, the optical density and biomass productivity were 3.7- and 5-fold higher than their corresponding values under autotrophic culture conditions, respectively. Moreover, T. chuii synthesized more polysaccharides and total lipids under mixotrophic conditions. In addition, T. chuii cultured under mixotrophic conditions synthesized more types of fatty acids than autotrophic culture conditions. At a C：N ratio of 16, the percentage of C 16：0 and C 18：1 reached 30.08% and 24.65% of the total fatty acid （TFA） content, respectively. These findings may provide a basis for large- scale mixotrophic culture of T. chuii, as a potential bait-microalga.

关 键 词：bait-microalga C：N ratio mixotrophic culture Tetraselmis chuii 

分 类 号：S963.213[农业科学—水产养殖]