奶牛胎衣不下与胎儿胎盘中血管紧张素Ⅱ受体1表达的相关性  

Relationship between retained fetal membranes and cows to expression of angiotensinⅡ type 1 receptor in placenta

作　　者：陈伟毅[1] 刘春杨[2] 李得鑫 周霄楠 姜国均[1] 

机构地区：[1]河北农业大学动物医学院,河北保定071000 [2]河北农业大学动物科技学院,河北保定071000

出　　处：《中国兽医科学》2017年第2期263-267,共5页Chinese Veterinary Science

基　　金：河北农业大学人才振兴学科计划项目(1081005)

摘　　要：通过对胎儿胎盘中血管紧张素Ⅱ受体1(AT1-R)m RNA相对表达量的比较,分析奶牛胎衣不下(RFM)与AT1-R表达的相关性,探讨奶牛胎衣不下的发生机制。将奶牛分为RFM组与胎衣正常脱落(NRFM)组,利用RT-PCR将奶牛胎儿胎盘中AT1-R基因进行扩增,采用实时荧光定量PCR技术(RT-qPCR)检测RFM和NRFM的奶牛胎儿胎盘组织中AT1-R基因m RNA相对表达水平的差异。结果显示,扩增后的AT1-R基因片段与Genbank中登录的核苷酸序列同源性为100%,奶牛RFM组的AT1-R基因m RNA转录水平明显低于奶牛NRFM组(P<0.01)。结果表明,AT1-R基因m RNA的转录水平与RFM的发生密切相关。To analyze the relationship between retained fetal membranes（RFM） of cows and the expression of angiotensinⅡtype 1 receptor（AT1-R） and explore the mechanism of onset of RFM,cows were divided into retained fetal membranes group and no retention of fetal membranes（NRFM） group,and the AT1-R genes in placenta were amplified by RT-PCR.Then the difference of the relative expression of AT1-R m RNA in the placenta tissue between RFM and NRFM was detected by real-time fluorescent quantitative PCR（RT-qPCR）.The results indicated that the AT1-R genes were cloned,of which the homologies of nucleotide sequences were 100% to those reported in Genbank,and the transcription level of AT1-R m RNA in RFM group was significantly lower than those in NRFM group（P〈0.01）.The results demonstrated that the transcription level of AT1-R m RNA was closely related to the onset of RFM.

关 键 词：胎衣不下 血管紧张素Ⅱ受体1 实时荧光定量PCR 

分 类 号：S857.2[农业科学—临床兽医学]