左卡尼汀通过激活Nrf2／HO-1途径减轻大鼠肝脏热缺血再灌注损伤  

L-carnitine alleviates liver warm ischemia-reperfusion injury in rats through activating Nrf2/HO-1 pathway

作　　者：李文川[1] 高良奎 李浩航 杨洁[1] 韦秋业 许佐明[1] 罗宗将 汪建初[2] 浦涧[2] 

机构地区：[1]右江民族医学院研究生学院,百色533000 [2]右江民族医学院附属医院肝胆外科

出　　处：《中华肝胆外科杂志》2017年第2期121-125,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：广西研究生教育创新计划项目（YCSZ2015225）

摘　　要：目的研究左卡尼汀对大鼠肝脏热缺血再灌注损伤（WIRI）的保护作用，探讨其作用机制。方法将24只雄性SD大鼠随机分为三组：假手术（SO）组、肝热缺血及再灌注（WIR）组、左卡尼汀预处理（LC）组。LC组腹腔注射左卡尼汀，SO组及WIR组注射等量生理盐水。检测血清ALT和AST水平，测定肝组织超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量，观察肝组织病理学改变，检测肝组织转录因子Nrf2和HO-1的mRNA表达。结果与SO组比较，WIR组血清ALT、AST水平明显升高（P〈0．05）；肝组织匀浆SOD活性明显降低，分别为（66．6±12．3）U／mgprot、（25．1±9．0）U／mgprot（P〈0．05）；肝组织匀浆MDA含量明显增加，分别为（1．5±0．3）nmol／mgprot、（3．6±0．8）nmol／mgprot（P〈0．05）；肝组织Nrf2、HO-1的mRNA相对含量明显增加（P〈0．05）。与WIR组比较，LC组血清ALT、AST均明显降低（P〈0．05）；肝组织匀浆SOD活性明显升高，分别为（25．1±9．0）U／mgprot、（86．0±11．4）U／mgprot（P〈0．05）；肝组织匀浆MDA含量明显减少，分别为（3．6±0．8）nmol／mgprot、（1．4±0．2）nmol／mgprot（P〈0．05）；肝组织Nrf2、HO一1的mRNA相对含量明显升高（P〈0．05）。结论左卡尼汀可减轻大鼠肝脏WIRI。这种保护作用可能是通过激活Nrf2／HO-1通路来实现。Objective To investigate the protective effects of L-carnitine on liver warm ischemiareperfusion injury （WIRI） in rats and to explore its possible role of L-carnitine in affecting Nrf2/HO-1 pathway. Methods Twenty-four male Sprague-Dawley （SD） rats were randomly divided into three groups including sham-operated （SO） group, liver warm ischemia and reperfusion （WIR） group and L-carnitine pretreatment （LC） group. L-carnitine was injected intraperitoneally in LC group while the equal volume of saline was injected in SO group and WIR group. The levels of ALT and AST in serum as well as the activities of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in liver tissues were detected. Meanwhile, pathologic change of liver tissue was observed. And then, the mRNA expression of NF-E2-related factor 2 （Nrf2） and heme oxygenase 1 （ HO-1 ） in liver tissues was determined. Results Compared with the SO group, ALT and AST level in serum were significantly increased （P 〈 0.05 ） in the WIR group; the activity of SOD in liver tissues was significantly reduced in the WIR group [ （66. 6 ± 12.3 ） U/mgprot vs. （25.1 ± 9.0） U/mgprot, P 〈 0.05 ] ; and the content of MDA in liver tissues was significantly increased in the WIR group [ （ 1.5 ± 0.3 ） nmol/mgprot vs. （ 3.6 ± 0.8 ） nmol/mgprot, P 〈 0.051 ; the mRNA expres- sion of Nrf2 and HO-1 were significantly increased （ P 〈 0.05 ） in the WIR group. Compared with the WIR group, ALT and AST level in serum were significantly reduced （P 〈 0.05） in the LC group; the activity of SOD in liver tissues was significantly increased in the LC group [ （ 25.1 ± 9.0） U/mgprot vs. （ 86. 0 ±11.4） U/mgprot, P 〈 0.05 ） ] ; and the content of MDA in liver tissues was significantly reduced in the LC group [ （3.6 ± 0.8） nmol/mgprot vs. （ 1.4 ± 0.2） nmol/mgprot, P 〈 0.05 ] ; the mRNA expression of Nrf2 and HO-1 were significantly increased （P 〈 0.05） in the LC group. Conclusion L-carn

关 键 词：左卡尼汀 肝热缺血再灌注损伤 NF-E2相关因子2 血红素加氧酶1 氧化 应激 

分 类 号：R657.3[医药卫生—外科学]