Sequence-based genetic mapping of Ds-tagged insertions to characterize malting-related traits in barley  

作　　者：Surinder Singh Jaswinder Singh 

机构地区：[1]Department of Food and Bioproduct Sciences, University of Saskatchewan 51 Campus Drive [2]Plant Science Department,21 111 Rue Lakeshore,St Anne de Bellevue,Mc Gill University

出　　处：《The Crop Journal》2017年第1期11-20,共10页作物学报（英文版）

基　　金：Funding for this project was provided by Barley Malting and Brewing Research Institute (grant number: 217248)

摘　　要：Among various functional genomics tools used to characterize genes in plants, transposonbased mutagenesis approaches offer great potential, especially in barley and wheat, which possess large genomes and in which genetic transformation is not routine. Two Ds transposon flanking sequences(TNPs), TNP-29(27.4 c M(centi Morgan)) and TNP-79(70.3 c M), were mapped in the vicinity of a malting quality QTL located on chromosome 4H of barley. Reactivation of the Ds transposon sequence from these TNP lines led to the identification of genes in the malting QTL regions. Several Ds(dissociation) lines were generated by crossing TNP-29 and TNP-79 with an Ac TPase(activator) expressing line(25-B), and F2 progenies were subsequently screened for Ds insertions at new locations. To further characterize these Ds mutants, we mapped the new Ds flanking sequences on a barley genetic map and found that 29% of Ds were located in regions associated with the malting QTL located on chromosome 4H and in close proximity to other important malting-associated QTL across the barley chromosome. Using a sequence based approach, a linkage map was generated that confirmed the position of Ds loci in the barley genome map. Locating these Ds loci on the barley map opens avenues to dissect important malting QTL for facilitating identification of candidate malting genes.Among various functional genomics tools used to characterize genes in plants, transposonbased mutagenesis approaches offer great potential, especially in barley and wheat, which possess large genomes and in which genetic transformation is not routine. Two Ds transposon flanking sequences（TNPs）, TNP-29（27.4 c M（centi Morgan）） and TNP-79（70.3 c M）, were mapped in the vicinity of a malting quality QTL located on chromosome 4H of barley. Reactivation of the Ds transposon sequence from these TNP lines led to the identification of genes in the malting QTL regions. Several Ds（dissociation） lines were generated by crossing TNP-29 and TNP-79 with an Ac TPase（activator） expressing line（25-B）, and F2 progenies were subsequently screened for Ds insertions at new locations. To further characterize these Ds mutants, we mapped the new Ds flanking sequences on a barley genetic map and found that 29% of Ds were located in regions associated with the malting QTL located on chromosome 4H and in close proximity to other important malting-associated QTL across the barley chromosome. Using a sequence based approach, a linkage map was generated that confirmed the position of Ds loci in the barley genome map. Locating these Ds loci on the barley map opens avenues to dissect important malting QTL for facilitating identification of candidate malting genes.

关 键 词：Transposon tagging Linkage mapping Malting QTL BARLEY 

分 类 号：S512.31[农业科学—作物学]