栎黄枯叶蛾幼虫寄主选择机制的光谱学研究  

Spectroscopic Studies on the Host Plant Selection Mechanism of Trabala vishnou gigantina Yang (Lepidoptera:Lasiocampidae)

作　　者：温冬梅[1] 陆鹏飞[1] 骆有庆[1] 周金池[2] 高永伟[2] 

机构地区：[1]北京林业大学林木有害生物防治北京市重点实验室,北京100083 [2]北京林业大学公共分析测试中心,北京100083

出　　处：《光谱学与光谱分析》2017年第2期541-544,共4页Spectroscopy and Spectral Analysis

基　　金：国家"十二五"科技支撑计划项目(2012BAD19B07);国家自然科学基金项目(31270693;31570643)资助

摘　　要：栎黄枯叶蛾(Trabala vishnou gigantina Yang)是一种多寄主林业害虫,在我国常造成多种经济作物的减产甚至绝收。为了对栎黄枯叶蛾幼虫的寄主选择机制进行深入了解,该研究在室内条件下,选取刺槐、柠条、旱柳、山杨、山杏和沙棘六种植物,采用双向选择实验方法,研究了栎黄枯叶蛾幼虫对六种植物的取食偏好性。并利用傅里叶变换红外光谱(FTIR)和X射线衍射(XRD)对六种供选植物的化学成分和物理结构进行了光谱学研究。研究结果表明,当寄主沙棘与山杏、山杨、旱柳、柠条、刺槐存在时,幼虫比较倾向于选择沙棘。栎黄枯叶蛾幼虫对六种植物的取食偏好性的顺序依次为:沙棘>山杏>旱柳>山杨>刺槐>柠条。FTIR谱图显示,六种植物在光谱特征吸收峰的位置、数目及形状上基本相似,差异不明显。通过对三个FTIR吸光度比率的分析后发现:植物中的脂类和多糖含量是影响幼虫取食选择的关键因素。通过XRD检测,测得了六种供试植物的结晶度。通过对结晶度变化趋势和幼虫取食偏好性的对比发现:结晶度可能一定程度上影响植物的适口性,进而影响了幼虫对寄主植物的取食偏好。该研究是光谱学分析方法首次应用于昆虫寄主选择机制的研究,将为深入研究昆虫与植物在分子层面上的互相影响开辟蹊径。Trabala vishnou gigantina Yang（Lepidoptera：Lasiocampidae）is a polyphagous forestry pest whose periodic breaking out results in great economic damage including total crop failure to forestry and fruit production in China.In this study,in order to improve the understanding of the host plant selection mechanism of T.vishnou gigantinalarvae,locust,caragana,willow,poplar,apricot and sea-buckthorn were used as potential host plants for the test.Two-way choice experiment method was used to study the T.vishnou gigantina Yang feeding preferences of the six kinds of plants.Moreover,the chemical component and physical structure of six plants were analyzed with Fourier transform infrared（FTIR）spectroscopy and X-ray diffraction（XRD）.Among the six plants,T.vishnou gigantinalarvae showed a strong preference for sea-buckthorn,followed by,apricot,willow,poplar,locust,and caragana.The FTIR analysis displayed that those six plants presented similar characteristic on absorption peak position,peak amount,and shape.The targets（1 154/1 733,1 154/898）by FTIR showed that lipids and polysaccharide were major nutriments to affect the host plant selection of T.vishnou gigantinalarvae.The XRD results showed that crystallinity index（CrI）also could affect the host plant selection of T.vishnou gigantinalarvae.In this research,spectroscopy technology was firstly applied to the study of interactive relationship between insect and host,which would blaze a trail for intensive study of host plant selection mechanism of insect at molecular level.

关 键 词：栎黄枯叶蛾 寄主选择 傅里叶红外光谱 X射线衍射 

分 类 号：O657.3[理学—分析化学]