解毒保肝方对急性药物性肝损害模型小鼠的影响  

Effects of Jiedu Baogan Decoction on Mice Model of Acute Drug- induced Liver Injury

作　　者：吴素芹[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《山东中医药大学学报》2017年第1期88-90,共3页Journal of Shandong University of Traditional Chinese Medicine

摘　　要：目的 :研究解毒保肝方治疗急性药物性肝损害的作用机制。方法 :小鼠随机分为空白对照组、模型组、水飞蓟宾葡甲胺组、解毒保肝方组,每组10只。空白对照组、模型组小鼠以生理盐水灌胃,水飞蓟宾葡甲胺组小鼠以水飞蓟宾葡甲胺片混悬液灌胃,解毒保肝方组小鼠以解毒保肝方水煎液灌胃,每日灌胃1次,共7次。末次灌胃后2 h,空白对照组以生理盐水0.1 m L/10 g灌胃,模型组、水飞蓟宾葡甲胺片组、解毒解毒保肝方组以40 mg/m L乙酰氨基酚片(AP)溶液0.1 m L/10 g灌胃,灌胃后禁食,不禁水。24 h后称重处死,检测血清丙氨酸氨基转氨酶(ALT)、门冬氨酸氨基转氨酶(AST)、蛋白浓度、肿瘤坏死因子α(TNF-α)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)水平。结果:与模型组比较,解毒保肝方可以降低AP所致急性药物性肝损害模型小鼠的ALT、AST,降低TNF-α活性,升高SOD,降低MDA,升高GSH。结论:解毒保肝方可能通过抑制过氧化反应及炎症反应,减少GSH消耗以对抗AP的肝毒性作用,且作用强于水飞蓟宾葡甲胺片。Objective：To study the mechanism of Jiedu Baogan decoction in the treatment of acute drug- induced liver damage. Methods：The mice were randomly divided into the blank control group,the model group,silibin meglumine group and Jiedu Baogan decoction group,10 in each group. The blank control group and model group were given by gavage with normal saline lavage,silibin meglumine group with silibin meglumine suspension and Jiedu Baogan decoction group with herbal decoction,one time daily for 7 times.Two hours after the last lavage,the blank control group was given by gavage with saline for 0.1 m L/10 g,the model group,silibin meglumine group and Jiedu Baogan decoction group with 40 mg/m L AP solution of 0.1 m L/10 g,fasting for solid but not for water. Twenty four hours later,the mice were executed and the corre sponding indexes such as the serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,protein concentration,tumor necrosis factor alpha（TNF- α）,superoxide dismutase（SOD）,glutathione（GSH）,malondialdehyde（MDA） levels were detected. Results ：Compared with the model group,Jiedu Baogan decoction can reduce the levels of ALT,AST,the activity of TNF alpha and the MDA,increase the levels of SOD and GSH in the model mice of acute drug- induced liver injury caused by AP. Conclusion：Jiedu Baogan decoction may reduce the consumption of GSH to against the hepatotoxic effects of AP by inhibiting the peroxide reaction and inflammations with better effect than silibin meglumine tablets.

关 键 词：解毒保肝方 急性药物性肝损害 水飞蓟宾葡甲胺片 小鼠 

分 类 号：R285.5[医药卫生—中药学]