葡萄糖氧化酶对生长獭兔营养物质利用、盲肠菌群结构及肠道消化酶活性的影响  

Effects of Glucose Oxidase on Nutrient Utilization,Cecal Microflora Structure and Intestinal Digestive Enzyme Activities of Growing Rex Rabbits

作　　者：刘亚娟[1,2] 陈赛娟[1,2] 李冲[3] 吴峰洋 高利晓 谷子林[2,3] 

机构地区：[1]河北农业大学山区研究所,保定071001 [2]河北省山区农业工程技术研究中心,保定071001 [3]河北农业大学动物科技学院,保定071001

出　　处：《动物营养学报》2017年第2期436-442,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家兔产业技术体系(CARS-44-05B);河北农业大学青年科学基金(QN201302)

摘　　要：本试验旨在研究葡萄糖氧化酶(GOD)对生长獭兔营养物质利用、盲肠菌群结构及肠道消化酶活性的影响。选取40日龄生长獭兔150只,随机分为5组,每组设5个重复,每个重复6只(公母各占1/2)。对照组饲喂基础饲粮,4个试验组饲喂在基础饲粮的基础上分别添加0.1%、0.2%、0.3%和0.4%GOD的试验饲粮。预试期5 d,正试期30 d。结果表明:1)能量的表观消化率和表观消化能以对照组最低,0.4%组最高;各试验组粗纤维表观消化率较对照组有不同程度提高,其中0.4%组提高16.10%,差异显著(P<0.05)。2)0.4%组可消化氮、氮沉积量、氮利用率和氮生物利用率分别较对照组提高12.39%、17.48%、15.68%和12.78%,且均差异显著(P<0.05)。3)饲粮中添加GOD能够在一定程度上降低盲肠p H。与对照组相比,0.4%组盲肠乳酸菌和双歧杆菌数量显著提高(P<0.05),大肠杆菌数量显著降低(P<0.05);0.3%组盲肠双歧杆菌数量显著提高(P<0.05)。4)0.4%组十二指肠部位胰蛋白酶活性显著高于对照组(P<0.05),空肠部位乳糖酶活性极显著高于对照组(P<0.01)。综合分析认为,饲粮中添加GOD能够改善饲粮中营养物质利用和獭兔肠道菌群结构,提高肠道消化酶活性,建议添加量为0.4%(60 U/kg)。This paper was aimed to study the effects of glucose oxidase（GOD） on nutrient utilization,cecal microflora structure and intestinal digestive enzyme activities of growing Rex rabbits.A total of 150 growing Rex rabbits with the age of 40 days were randomly assigned to 5 groups with 5 replicates per group and 6 rabbits per replicate（half male and half female）.The rabbits in control group were fed a basal diet,while the others in 4 experimental groups were fed the basal diet supplemented with 0.1%,0.2%,0.3%and 0.4%GOD,respectively.The trial lasted for 5 days for adaptation and 30 days for test.The results showed as follows：1）the apparent digestibility of energy and apparent digestible energy reached to the minimum in the control group,while reached to the maximum in the 0.4%group.Compared with the control group,crude fibre apparent digestibility of experimental groups were elevated more or less,and 0.4%group was significantly increased by16.10%（P〈0.05）.2） Compared with the control group,the digestible nitrogen,nitrogen retention,nitrogen utilization and nitrogen bio availability of 0.4%group were significantly increased by 12.39%,17.48%,15.68%and 12.78%,respectively（P〈0.05）.3） GOD addition could decrease the pH in caecum to a certain degree.Compared with the control group,the amounts of Lactobacilli and Bifido bacterium in caecum of 0.4%group were significantly increased（P〈0.05）,while the amount of Escherichia coli was significantly reduced（P〈0.05）;moreover,the amount of Bifidobacterium in caecum of 0.3%group was significantly increased（P〈0.05）.4） Compared with the control group,the trypsin activity in duodenum of 0.4%group was significantly increased（P〈0.05）,and the lactase activity in jejunum was significantly increased（P〈0.01）.By synthesize analyses,diets with GOD can improve the intestinal microflora structure,digestive enzyme activities and nutrient utilization of Rex rabbits.Addition of 0.4%（60 U/kg） GOD is recommended.

关 键 词：葡萄糖氧化酶 营养物质利用 菌群结构 消化酶活性 

分 类 号：S816[农业科学—饲料科学]