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作 者:陈娜娜[1] 何俊娜[1] 郭阳[1] 刘兵[1] 熊平文 余东游[1]
机构地区:[1]浙江大学动物科学学院,农业部华东动物营养与饲料重点实验室,杭州310058
出 处:《动物营养学报》2017年第2期511-518,共8页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:农业部饲料质量安全监管专项(2014);东南沿海地区大学农业科技服务技术集成与示范(2013BAD20B02)
摘 要:本试验旨在研究饲粮中添加高剂量蛋氨酸锌对蛋鸡生产性能、血液学指标和组织病理学的影响,以确定蛋鸡对饲用蛋氨酸锌的耐受剂量。试验采用单因子完全随机设计,选择健康且体重和产蛋率相近的20周龄海兰白蛋鸡540只,随机分成6个组,每个组6个重复,每个重复15只鸡。各组分别饲喂蛋氨酸锌添加水平为0(对照)、70(有效剂量)、140(2倍有效剂量)、350(5倍有效剂量)、700(10倍有效剂量)和1 400 mg/kg(20倍有效剂量)的试验饲粮。试验为期8周。结果显示:1)与对照组相比,70 mg/kg组、140 mg/kg组日产蛋重和产蛋率显著提高(P<0.05),料蛋比显著降低(P<0.05);350 mg/kg组、700 mg/kg组上述指标与对照组差异不显著(P>0.05);与0~700 mg/kg组相比,1 400 mg/kg组日产蛋重和产蛋率极显著降低(P<0.01),料蛋比极显著提高(P<0.01)。2)饲粮中添加蛋氨酸锌对蛋鸡血液常规指标无显著影响(P>0.05)。3)与对照组相比,饲粮中添加70~700 mg/kg蛋氨酸锌对蛋鸡血清生化指标无显著影响(P>0.05),但添加1 400 mg/kg蛋氨酸锌极显著提高了血清葡萄糖、尿酸、肌酐、总胆红素含量以及谷草转氨酶、碱性磷酸酶活性(P<0.01)。4)添加20倍推荐有效剂量(1 400 mg/kg)的蛋氨酸锌会引起主要器官组织的损伤和病变。结果提示,蛋鸡饲粮中蛋氨酸锌的耐受剂量为700mg/kg。This experiment was conducted to determine the tolerant dosage of zinc-methionine supplementation in diet through evaluating its effects on performance, hematology indices and histopathological changes of laying hens. Five hundred and forty healthy 20-week-old Hy-Line white laying hens with similar body weight and laying rate were randomly allocated into 6 groups with 6 replicates per group and 15 birds per replicate. Birds were fed basal diets supplemented with 0 (control), 70 (the recommended available dose, RAD), 140 (two folds of RAD ), 350 ( five folds of RAD ), 700 ( ten folds of RAD ) and 1 400 mg/kg ( twenty folds of RAD) zinc-methionine, respectively. The trial lasted for 8 weeks. The results showed as follows : 1 ) compared with the control group, daily egg mass and laying rate were significantly increased and the ratio of feed to egg was significantly reduced in 70 and 140 mg/kg groups (P〈0.05) ; there was no significant difference between 350 or 700 mg/kg groups and control group in the above indices (P〉0.05) ; compared with 0 to 700 mg/kg zinc-methionine, dietary supplemented with 1 400 mg/kg zinc-methionine significantly reduced daily egg mass and laying rate (P〈0.01), and increased the ratio of feed to egg (P〈0.01). 2) Dietary zinc-methionine supplementation had no significant effects on blood routine parameters (P〉0.05). 3) Compared with the control group, dietary supplemented with 70 to 700 mg/kg zinc-methionine had no significant effects on serum bio- chemical indices (P〉0.05), however, supplemented with 1 400 mg/kg zinc-methionine significantly in- creased the contents of glucose, uric acid, creatinine and total bilirubin and improved the activities of glutamicoxalacetic transaminase and alkaline phosphatase (P〈0.01). 4) Dietary supplementation with twenty folds of RAD (1 400 mg/kg) zinc-methionine damaged major organ tissues. It is indicated that the tolerant dosage of zinc-methionine in laying hens diet is 700 mg/
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