转CP4-EPSPS基因耐草甘膦除草剂大豆中作J9331喂养鹌鹑90d亚慢性毒理学研究  

A 90 d Subchronic Feeding Study of CP4-EPSPS Transgenic Glyphosate Herbicide-resistant Soybean(Glycine max) ZZ-J9331 in Quails(Coturnix japonica)

作　　者：刘燕[1] 章嫡妮[1] 于赐刚[1] 王长永[1] 卞慧敏[2] 

机构地区：[1]环境保护部南京环境科学研究所/国家环境保护生物安全重点实验室,南京210042 [2]南京中医药大学药学院,南京210023

出　　处：《农业生物技术学报》2017年第3期451-460,共10页Journal of Agricultural Biotechnology

基　　金：国家转基因生物新品种培育科技重大专项(No.2016ZX08012-005;2014ZX08012-005和No.2016ZX08011-003;2014ZX08011-003)

摘　　要：随着转基因耐草甘膦除草剂大豆(Glycine max)的迅速推广及扩大种植,其环境安全性也越来越成为人们关注的焦点。本研究选取与环境密切相关的鸟类-朝鲜鹌鹑(Coturnix japonica)作为实验动物,随机分成5个处理组,用含有28%和70%的耐草甘膦转基因大豆(中作J9331)和非转基因对照大豆(中黄30)以及常规基础日粮饲喂鹌鹑90 d,观察其对鹌鹑健康状况和生理指标的影响,实验期间,记录各组动物体重和摄食量,实验末期,收集血液解剖动物进行病理观察,计算脏器系数。结果显示,转基因大豆喂养动物90 d后,各组动物生长发育良好,转基因大豆与非转基因大豆对照组相比,鹌鹑的血常规、血生化和脏器系数等个别指标有统计学差异(P<0.05),但并无生物学意义。病理检查中,各实验组与基础日粮对照组相比,其他脏器未观察到有明显的病理改变,但在肝脏切片中观察到肝细胞脂肪变性及弥漫性纤维结缔组织增生,经评分证实该现象同时出现在实验组和基础日粮对照组,因此认为是肝脏自发性病变,与鹌鹑是否摄取转基因大豆无关。研究表明,转基因耐草甘膦除草剂大豆中作J9331与非转基因大豆对鹌鹑具有同等的食用安全性,为转基因大豆的商业化应用和安全管理提供了科学数据资料。Transgenic herbicide-resistant soybean （Glycine max） is one of the most widely planted genetically modified crops in the world. Meanwhile, the impacts of its rapid extension brought to environmental have become a focus of public interests. In this study, the Korean quails （Coturnix japonica） were selected as test animals, which were closely related to the environment, were randomly divided into 5 treatment groups. To assess the safety of transgenic herbicide-resistant soybean to birds, a 90 d feeding trial with transgenic soybean （ZZ-J9331） and its non-transgenic counterpart （ZH-30） were supplied to quails in basal feed with a ratio of 28% and 70% respectively. After 90 d feeding, the influence of genetically modified （GM） soybean were evaluated by monitoring quails health and physiological indexes, recording animal＇s body weight and food intake, dissecting animals and calculating viscera coefficient afterwards. It was observed after 90 d, animals of each group were in good condition. Quails in each group ate normal, moved freely, and had shiny hair, nose, eyes and mouth without abnormal secretions. The body weight of all experimental groups showed an increasing trend, and no abnormal changes in food intake. Different from the previous literature, in addition to the above related to the growth and development index, this study collected blood samples from quail at the end of the experiment, blood and blood biochemical detection, in addition, this study also had some important organs pathological dyeing processing, in order to observe the possible toxicity of glyphosate tolerance of transgenic soybean on quails from the toxicological perspective. Compared with the non-GM soybean control group, several indexes of the serum biochemistry, hematology, relative organ weight of quails had statistically significant difference （P〈0.05） and no dose-relative or gender-relative, indicating no obvious biological significance. Moreover, no pathological change was observed in heart, brain, kidney, lu

关 键 词：耐草甘膦除草剂 转基因大豆 朝鲜鹌鹑 90 d喂养实验 

分 类 号：S565.1[农业科学—作物学]