FGL2在急性病毒感染过程中对T细胞免疫应答的影响研究  

FGL2 is dispensable for T-cell response during acute viral infection

作　　者：谢东京 黄煌[1] 周新元[1] 

机构地区：[1]第三军医大学全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2017年第3期197-202,共6页Immunological Journal

基　　金：国家自然科学基金(31470870)

摘　　要：目的探讨纤维蛋白原样2凝血酶原酶(FGL2)在急性病毒感染过程中对T细胞的增殖、分化和功能的影响。方法模型一:淋巴细胞脉络丛脑膜炎病毒(LCMV)感染Fgl2基因敲除小鼠(实验组)与C57BL/6J对照小鼠,感染后第8天,检测T细胞的增殖、分化和功能。模型二:过继转移能够特异性识别LCMV的TCR转基因小鼠SMARTA或P14的T细胞到Fgl2基因敲除小鼠(实验组)和C57BL/6J对照小鼠内,LCMV感染后第8天,检测小鼠体内供体SMARTA或P14细胞的增殖和分化。结果FGL2在LCMV急性感染过程中表达上调(P<0.05);实验组和对照组的滤泡辅助T细胞(T_(FH))、Th1细胞和效应CD8^+T细胞的频率和数量均无明显差异(P>0.05);GC B细胞和浆细胞的数量和频率、LCMV特异性抗体的滴度以及效应CD8^+T细胞中表达TNF-α和CD107a/b的频率均无明显差异(P>0.05)。结论 FGL2在急性病毒感染过程中不影响T细胞的免疫应答。It was reported that fibrinogen-like protein 2（FGL2） was induced during MHV-3 infection, but itsfunction for T-cell response remains unclear. Here we showed that FGL2 was induced during acute LCMV infection,according to the previous reports. But FGL2 knock-out mice, compared with C57BL/6J mice, exhibited consistentT-cell response upon LCMV infection, including similar frequency and quantity of T（FH）cells, Th1 cells, effecter CD8^＋T cells, GC B cells and plasma cells. Together, our data suggest that FGL2 is dispensable for T-cell response duringacute viral infection and the induction of FGL2 merely represents a nonfunctional marker of T-cell response.

关 键 词：纤维蛋白原样2凝血酶原酶 滤泡辅助T细胞 TH1细胞 效应CD8+T细胞 

分 类 号：R392[医药卫生—免疫学]