广谱抗源H4中2个主效抗病基因的单基因系构建及评价  

Development of near-isogenic lines for two blast resistance genes in an indicarice H4

作　　者：孙大元[1] 周丹华[2] 张景欣[1] 王慧[2] 朱小源[1] 杨祁云[1] 陈志强[2] 

机构地区：[1]广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,广东广州510640 [2]华南农业大学国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《江苏农业学报》2017年第1期1-5,共5页Jiangsu Journal of Agricultural Sciences

基　　金：广东省自然科学基金重点项目(2016A030311017);"十二五"国家"863"计划项目(2012AA101201);国家自然科学基金项目(31401722)

摘　　要：近等基因系是准确鉴定病原菌无毒基因型,监测病原菌群体无毒基因结构动态变化以及开展寄主抗病性遗传研究与抗病育种应用的重要材料。为了明确广谱抗源H4中2个主效抗病基因Pik-H4和Pita-H4与已知等位基因之间的抗性差异,以丽江新团黑谷(LTH)为背景,采用分子标记辅助选择技术和抗性评价等方法,构建广谱抗源H4的2个主效抗病基因Pik-H4和Pita-H4的近等基因系。结果表明:携带Pik-H4的近等基因系的抗性明显好于携带Pita-H4的近等基因系。Near-isogenic lines（ NIL） are expected to be a valuable genetic resource for blast resistance,monitoring blast races and revealing their pathogenicities. To understand resistance difference between Pik-H4,Pita-H4 and their allelic gene,we constructed two near-isogenic lines with LTH as background by combining marker-assisted selection with resistance evaluation in this study. It was found that the resistance of NIL carrying Pik-H4 was better than that carrying Pita-H4.

关 键 词：稻瘟病抗性基因 分子标记辅助选择 近等基因系 

分 类 号：S332.2[农业科学—作物遗传育种]