鸡源大肠杆菌Bla_(CTX-M-125)型ESBL基因的定位及遗传环境  

The location and genetic environment of Bla_(CTX-M-125) ESBL gene from Escherichia coli in chickens

作　　者：王翔宇[1] 宋玉慧[1] 荆炜[1] 朱晓清[1] 徐倩[1] 杜向党[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450003

出　　处：《江苏农业学报》2017年第1期141-145,共5页Jiangsu Journal of Agricultural Sciences

基　　金：河南省高等学校重点科研项目(14A230008)

摘　　要：采用PCR扩增、测序分析、接合试验、药敏试验、脉冲场凝胶电泳(PFGE)和Southern blot方法,分析了1株兽医临床分离大肠杆菌(sd-3)携带的超广谱β-内酰胺酶(ESBL)基因的状况、分型、定位及遗传环境。结果显示,该株大肠杆菌携带ESBL基因,属于bla_(CTX-M-9)亚群的Bla_(CTX-M-125)亚型,系在大肠杆菌中首次检出;接合试验、PFGE和Southern blot证实该基因位于接合型质粒上;Bla_(CTX-M-125)基因的上、下游分别存在插入序列ISEcp1和IS903。这表明插入序列和接合型质粒均可在大肠杆菌Bla_(CTX-M-125)基因的水平转移中发挥重要作用,有必要在肠杆菌科细菌中开展该基因的监视。The presence,types,location and genetic environment of extended-spectrum β-lactamases（ ESBLs） gene in a Escherichia coli strain（ sd-3） isolated from veterinary clinic were investigated by PCR amplification,sequencing analysis,conjugation assay,antimicrobial susceptibility test,pulsed field gel electrophoresis（ PFGE） and Southern blot. The results showed the strain carried Bla_（CTX-M-125） ESBLs,belonging to the blaCTX-M-9subgroup,which was firstly described in Escherichia coli. Conjugation,PFGE and Southern blot confirmed that the gene was located on the conjugative plasmid. The upstream and downstream of the Bla_（CTX-M-125） was flanked by insertion sequence ISEcp1 and IS903,respectively. This indicated that both insertion sequence and conjugative plasmid had played an important role in the horizontal transfer of the Bla_（CTX-M-125） ESBL gene. The surveillance of the Bla_（CTX-M-125） in enterobacteriaceae bacteria is necessary.

关 键 词：Β-内酰胺酶 大肠杆菌 blaCTX-M-125 接合型质粒 

分 类 号：S851.347.202[农业科学—预防兽医学]