黄瓜RIL群体侧枝相关性状QTL定位  

Genetic mapping of QTL associated with lateral branch-related traits using recombinant inbred lines population in cucumber( Cucumis sativus L.)

作　　者：张微微[1,2] 潘俊松[2] 蒋苏[2] 何欢乐[2] 俞平高[1] 蔡润[2] 

机构地区：[1]上海农林职业技术学院园艺园林系,上海201699 [2]上海交通大学农业与生物学院,上海200240

出　　处：《江苏农业学报》2017年第1期174-180,共7页Jiangsu Journal of Agricultural Sciences

基　　金：上海市自然科学基金项目(15ZR1429800)

摘　　要：利用侧枝长势弱、萌发早的华北类型品系S94和侧枝长势强、萌发晚的欧洲类型品系S06构建的224个F6:7代重组自交系(RIL)群体,对春、秋两季F7黄瓜侧枝相关性状表型进行研究。利用重组自交系群体构建遗传图谱,采用Win QTLcart2.5软件复合区间作图法,对4个侧枝相关性状[侧枝总长(lbtl)、侧枝均长(lbal)、侧枝数目(lbn)和第一侧枝节位(flbn)]QTL位点进行检测。结果显示,4个侧枝相关性状在秋、春两季共检测到45个QTLs位点(秋季29个,春季16个),分布于全部7条染色体上。在侧枝相关性状中,春、秋两季相同性状都能稳定检测到的QTL有8个,其中2个QTL侧枝均长QTL lbal1.1(秋季贡献率16.4%,春季贡献率13.5%)和侧枝总长QTL lbtl1.1(秋季贡献率21.2%,春季贡献率26.9%)在两季的贡献率都高于10%,其余位点的贡献率(>10%)都在单一季节表现。A set of 224 recombinant inbred lines（ RILs） were derived from a cross between S94（ Northern China type with weak lateral branch growth potential and early lateral branch sprouting time） and S06（ Northern European type with strong lateral branch growth potential and late lateral branch sprouting time）. The phenotypic data in different seasons（ F7 in spring and F7 in autumn） were used to identify the quantitative trait loci for four lateral branch-related traits（ lateral branch total length（ lbtl）,lateral branch average length（ lbal）,lateral branch number（ lbn）,and first lateral branch node（ flbn）） using Win QTLcart2.5 software.The results showed a total of 45 QTLs for lateral branch-related traits were detected in F7 in autumn（ 29） and spring（ 16）,and the QTLs distributed in all seven chromosomes. Of all lateral branch-related traits,QTLs of same traits detected both in spring and autumn were eight,of which lateral branch average length lbal1. 1（ 16.4% in autumn,13.5% in spring） and lateral branch total length lbal1.1（ 21.2% in autumn,26.9% in spring） had stable position and higher contribution in both seasons,and the rest of loci showed high contribution（ 〉10%） in a single season.

关 键 词：黄瓜 遗传图谱 侧枝 QTL 

分 类 号：S642.2[农业科学—蔬菜学]