CRISPR/Cas系统最新研究进展  

The Latest Research Progress on CRISPR/Cas System

作　　者：刘妮[1] 陆沁[1] 刘娟[1] 陈航[1,2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224 [2]国家林业局资源昆虫培育与利用重点实验室,昆明650224

出　　处：《生物技术通报》2017年第2期53-58,共6页Biotechnology Bulletin

基　　金：国家林业局948项目(2014-4-66);云南省科技计划面上项目(2013FB087)

摘　　要：CRISPR/Cas系统为细菌与古细菌中抵御外源病毒和质粒DNA入侵的获得性免疫机制系统。作为一种新型的基因组编辑技术,具有设计简单、特异性强、效率高等优点,为基因组定向改造调控和应用带来了突破性的革命。就CRISPR/Cas系统的研究背景、存在类型以及TypeⅡ和TypeⅢCRISPR系统的基本作用原理和新的研究突破做了较为详尽的介绍,以便为读者和相关领域的研究者提供参考。The CRISPR/Cas is a system of immune mechanism for bacteria and archaea against exogenous virus and plasmid DNA invasion. As a novel genome editing technology, it possesses the advantages of a simple design, strong specificity, and high efficiency, bringing revolutionary breakthrough for genome directional modification, regulation and application. We introduce the research background, the existence type of the CRISPR/Cas system, and basic action mechanism of Type II and Type III CRISPR system and new research breakthrough in detailed, for providing the reference for readers and researchers in related fields.

关 键 词：基因组编辑技术 CRISPR/Cas系统 Cas9 scRNA 

分 类 号：Q78[生物学—分子生物学]