结缕草转录因子基因ZjDREB4.1克隆和逆境表达模式  

Cloning and Expression Profiles of a Transcription Factor Gene Zj DREB4.1 in Zoysia japonica Under Adversity

作　　者：李京[1] 吴奇[1] 张琳婕[1] 李旭婷[1] 周敏琪[1] 韦善君[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《生物技术通报》2017年第2期80-88,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31100507);国家大学生科学研究与创业行动计划项目(GCCX2016110016)

摘　　要：以日本结缕草(Zoysia japonica)‘Meyer’品种为材料,克隆获得了1个DREB(dehydration responsive element binding)类转录因子基因,命名为Zj DREB4.1。该基因编码区长651 bp,编码216个氨基酸,推测的蛋白质Zj DREB4.1分子量为22.9 k D,等电点p I为5.74,第50-110位氨基酸组成一个典型的AP2保守结构域。在基因的系统发生树中,Zj DREB4.1蛋白与拟南芥At TINY蛋白和玉米Zm DBF2蛋白聚为一支,属于DREB亚家族A-4组。在叶组织中Zj DREB4.1为组成型表达,受低温诱导上调表达,在干旱和高盐胁迫下表达先下调后恢复至正常水平。A novel DREB （ dehydration responsive element binding） transcription factor gene, designated as ZjDREB4.1, was isolated from cuhivar ＇ Meyer＇ of Zoysia japonica. The open reading frame （ ORF ） of ZjDREB4.1 was 651 bp in length, encoding 216 amino acid residues. The putative protein ZjDREB4.1 was 22.9 kD in molecular weight and with a theoretical isoelectric point （ pI ） of 5.74. Amino acids from the 50^th to the 110^th were predicted to build up a conserved AP2 domain. The phylogenetic tree analysis showed that ZjDREB4.1 was grouped with AtTINY ofArabidopsis thaliana and ZmDBF2 of maize, belonging to the A-4 group of DREB subfamily. ZjDREB4.1 was expressed constitutively in leaves. The expression was up-regulated under cold stress, and was first down-regulated then returned to normal level under drought and salt stresses.

关 键 词：转录因子 DREB 半定量RT-PCR 非生物胁迫 

分 类 号：Q943.2[生物学—植物学]