复方苦芩对细小病毒感染犬血液T细胞亚群和细胞因子的影响  

Effect of Compound Kuqin on T Cell Subsets and Cytokines of Blood in Canines with Canine Parvovirus

作　　者：刘娟[1] 吴秋月[1] 邱洪[1] 

机构地区：[1]西南大学荣昌校区动物医学系,荣昌402460

出　　处：《畜牧兽医学报》2017年第2期369-376,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31172357)

摘　　要：研究复方苦芩对细小病毒感染犬T细胞亚群和细胞因子的影响。建立犬细小病毒感染模型,把动物随机分为6个组,分别为空白对照组,模型组,阳性药物组,复方苦芩高、中、低剂量组,每组20只。将攻毒后6h设定为试验0d,分别于试验0、7、14、21、28、35d采血,流式细胞术检测T淋巴细胞亚群,试剂盒检测IL-2、IL-4、IFN-γ含量。结果显示:与模型组相比,复方苦芩可促进犬机体分泌IL-2、IL-4和IFN-γ,提高外周血CD3+T、CD3+CD4+T细胞百分数,降低CD3+CD8+T细胞百分数。复方苦芩能够调节细小病毒感染犬的免疫机能,提高犬抗病毒能力。In order to study the effect of compound Kuqin on T cell subsets and cytokines of blood in canines with canine parvovirus,we established the canine parvovirus model,and separate 120 canines into 6groups,like blank control group,model group,positive medicine group,compound Kuqin high dose group,middle dose group,low dose group,with 20 canines in each group.We set the time point that 6hours after the virus was inoculated as 0d,and collect the blood sampling from the canine saphenous vein at the day 0,day 7,day 14,day 21,day 28,and day 35.T lymphocyte subsets were detected by flow cytometry,and the cytokines IL-2,IL-4,IFN-γwere detected by kit.The result showed that,compared with the model group,compound Kuqin can promote the secretion of IL-2,IL-4,IFN-γ,and increase the percentage of CD3~＋,CD3~＋CD4~＋T cells in peripheral blood,and decrease the percentage of CD3~＋CD8~＋T cells.In conclusion,compound Kuqin can regulate canines＇ immune function and improve the ability of antiviral after they were infected by canine parvovirus.

关 键 词：苦芩 犬细小病毒 T细胞亚群 细胞因子 

分 类 号：S853.74[农业科学—临床兽医学]