半夏泻心汤含药血清对胃癌微环境诱导人腹膜间皮细胞氧化应激反应的影响  

Influence of Drug Serum in Pinellisa Decoction for Purging Stomach-fire on Peritoneal Mesothelial Cell Oxidative Stress by Gastric Cancer Microenvironment

作　　者：崔国宁 刘喜平[1] 李沛清[1] 明海霞[1] 张炜[2] 王强[1] 董俊刚[1] 杨柏林[1] 

机构地区：[1]甘肃中医药大学基础医学院,甘肃兰州730000 [2]兰州大学第一医院,甘肃兰州730000

出　　处：《时珍国医国药》2017年第1期12-14,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260525);甘肃省高校基本科研业务费专项基金(No.BH-2011-068)

摘　　要：目的观察半夏泻心汤含药血清对胃癌微环境诱导腹膜间皮HMr SV5细胞氧化应激反应的影响。方法将胃癌BGC-823细胞上清与人腹膜间皮HMr SV5细胞共培养,建立胃癌微环境对腹膜间皮细胞损伤模型,以100μl/ml、200μl/ml、400μl/ml浓度的半夏泻心汤含药血清进行干预,采用流式细胞术检测腹膜间皮HMr SV5细胞活性氧(ROS)水平、比色法检测腹膜间皮细胞HMr SV5超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)的量。结果经胃癌微环境诱导后腹膜间皮细胞中的ROS及MDA显著升高,SOD及GSH水平下降(P<0.05),经100μl/ml、200μl/ml、400μl/ml浓度的半夏泻心汤含药血清进行干预后,ROS及MDA水平显著降低,SOD及GSH水平显著升高(P<0.05),且呈浓度依赖关系。结论胃癌微环境可诱导人腹膜间皮HMr SV5细胞发生氧化应激反应,半夏泻心汤含药血清可抑制胃癌微环境诱导的腹膜间皮细氧化应激反应。Objective To observe the influence of drug serum in Pinellisa Decoction for purging stomach-fire on peritoneal mesothelial cell HMr SV5 oxidative stress by gastric cancer microenvironment. Methods Co-cultivate gastric cancer BGC-823 cellular supernatant and peritoneal mesothelium HMr SV5 cell to establish damage model of gastric cancer microenvironment to peritoneal mesothelial cells. Intervene with different concentrations of drug serum in pinellisa decoction for purging stomach-fire with a concentration of 100μl/ml,200μl/ml,400μl/ml respectively,observe HMr SV5 cell ROS levels by Flow cytometry method,detect content of peritoneal mesothelium cell HMr SV5 SOD,MDA,GSH by Colorimetry method. Results Gastric cancer microenvironment induced peritoneal mesothelium cell survival rate decreased,peritoneal mesothelium cell of ROS and MDA had significantly higher,SOD and GSH levels drop（ P 〈 0. 05）. After intervene with different concentrations of drug serum in pinellisa decoction for purging stomach-fire with a concentration of 100μl/ml,200μl/ml,400μl/ml respectively had concentration dependence relationship,ROS and MDA were significantly reduced,SOD and GSH levels drop（ P 〈 0. 05）. Conclusion Gastric cancer microenvironment can induce peritoneal mesothelium HMr SV5 cell oxidative stress reaction,drug serum in pinellisa decoction for purging stomach-fire inhibits gastric cancer microenvironment induced peritoneal mesothelium fine oxidative stress reaction.

关 键 词：氧化应激反应 胃癌微环境 腹膜间皮细胞 半夏泻心汤 

分 类 号：R285.5[医药卫生—中药学]