瑞香素对CIA大鼠脾T淋巴细胞杀瘤效应的影响  

Effects of Daphnetin on the spleen T lymphocyte tumor-cytotoxicity effect of CIA rats

作　　者：王洁莹[1] 刘玮[1] 郑茂[1] 况南珍[1] 曾小平[1] 傅颖媛[1] 

机构地区：[1]南昌大学基础医学院,江西南昌330006

出　　处：《时珍国医国药》2017年第1期33-35,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31460239)

摘　　要：目的探讨瑞香素对CIA大鼠脾T淋巴细胞杀瘤效应的影响。方法建立胶原诱导关节炎(CIA)大鼠模型。大鼠随机分为健康对照组、CIA模型对照组、瑞香素治疗组、阳性药物对照组,连续治疗28天后处死大鼠,分离脾细胞。MTT法测定Con A诱导大鼠脾T淋巴细胞增殖率。MTT法检测大鼠脾T淋巴细胞对瘤细胞(肝癌细胞株SMMC-7721)的杀伤率。结果 1瑞香素治疗组脾T淋巴细胞增殖率为(161.11±14.05)%,与CIA模型对照组(55.61±10.88)%、阳性药物对照组(13.00±12.14)%、健康对照组(101.35±16.79)%比均明显增高(P<0.05)。2瑞香素治疗组大鼠脾T淋巴细胞对瘤细胞杀伤率为(23.50±11.08)%,与CIA模型对照组(15.89±8.77)%、阳性药物对照组(7.74±8.84)%比均明显增高(P<0.05),接近健康对照组(24.35±13.45)%。结论瑞香素能明显提高CIA大鼠脾T淋巴细胞增殖率和脾T淋巴细胞对瘤细胞杀伤率,有效促进CIA大鼠免疫功能。Objective To investigate the effect of Daphnetin on spleen T lymphocyte tumor-cytotoxicity effect of CIA rats. Methods 1. Established collagen-induced arthritis（ CIA） rat model. The rats were randomly divided into healthy control group,CIA model group,Daphnetin treatment group,Positive drug control group. Continuous treatment for 28 days,the rats were sacrificed and spleen cells were isolated after the end of treatment. 2. Determined Con A induced spleen T lymphocyte proliferation rate by MTT assay. 3. Determined the suppression rate of CIA rats to tumor cells（ SMMC-7721） by MTT assay. Results 1. The spleen T lymphocyte proliferation rate of Daphnetin treatment group was 161. 11 ± 14. 05,compared with CIA model group（ 55. 61± 10. 88）,Positive drug control group（ 13. 00 ± 12. 14） and healthy control group（ 101. 35 ± 16. 79） showed marked difference（ P 〈 0. 05）. 2. The suppression rate to tumor cells of Daphnetin treatment group was 23. 50 ± 11. 08,compared with CIA model group（ 15. 89 ± 8. 77） and Positive drug control group（ 7. 74 ± 8. 84） showed marked difference（ P 〈 0. 05）,closed to the healthy control group（ 24. 35 ± 13. 45）. Conclusion Daphnetin can significantly improve the spleen T lymphocyte proliferation rate and the suppression rate to tumor cells of CIA rat,effectively promote the immune function of CIA rats.

关 键 词：瑞香素 CIA大鼠 脾T淋巴细胞 杀瘤效应 

分 类 号：R285.5[医药卫生—中药学]