石菖蒲挥发油有效成分联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠血脑屏障的影响  

Effect of the effective components of volatile oil of Acorus tatarinowii Schott and levodopa co-administration on blood brain barrier in 6-hydroxydopamine induced Parkinson's rat

作　　者：黄丽平[1,2] 马阮昕 冯真英 邓敏贞[5] 

机构地区：[1]海南医学院,海南海口571199 [2]岭南师范学院,广东湛江524048 [3]中山大学附属第六医院药学部,广东广州510655 [4]湛江市第二人民医院,广东湛江524003 [5]广东省中医院(广东省中医药科学院广州中医药大学第二临床医学院),广东广州510120

出　　处：《时珍国医国药》2017年第1期59-62,共4页Lishizhen Medicine and Materia Medica Research

基　　金：海南省自然基金项目(No.20168266);海南省科协青年科技人才学术创新计划项目(No.HAST201635);海南医学院科研培育基金项目(No.HY2015-01);2015年学校创新计划--优秀博士论文培养项目(No.A1-AFD018161Z01024)

摘　　要：目的研究石菖蒲挥发油有效成分联合左旋多巴对6-羟基多巴诱导帕金森病模型大鼠血脑屏障的影响。方法定位于SPF级SD大鼠左脑的内侧前脑束(前囟后:-4.4 mm,中线旁:-1.2 mm,硬膜下:-7.8 mm),注射24μg的6-羟基多巴,造模1个月后,将造模大鼠腹腔注射0.5 mg/kg的阿扑吗啡,大鼠向健侧旋转数大于7圈/min为成功PD模型。将40只PD模型大鼠随机分为4组,每组10只,雌雄各半,即模型组,左旋多巴组(60 mg/kg),β-细辛醚组(15 mg/kg),联合用药组(左旋多巴60 mg/kg+β-细辛醚15 mg/kg)。给药组按上述剂量灌胃给药,一天2次,给药30天。末次给药1 h后,冰上迅速分离皮质。高效液相色谱法(HPLC)检测左旋多巴和多巴胺含量;流式细胞术检测皮质紧密连接蛋白(Zonula occludens 1,ZO-1)、紧密连接相关蛋白(Occludin)和肌动蛋白(Actin)含量;免疫组化检测紧密连接相关蛋白5(Claudin-5)。结果与假手术组比较,模型组大鼠的左旋多巴和多巴胺含量都显著减少(P<0.05),ZO-1、Occludin、Actin和Claudin-5的表达无显著变化(P>0.05);与模型组比较,联合用药组大鼠的左旋多巴和多巴胺含量都显著增加(P<0.01),ZO-1、Occludin、Actin和Claudin-5的表达都显著减少(P<0.05);与左旋多巴组比较,联合用药组大鼠的左旋多巴和多巴胺含量显著增加(P<0.05),联合用药组大鼠的ZO-1、Occludin、Actin和Claudin-5的表达都显著减少(P<0.05)。结论石菖蒲挥发油有效成分β-细辛醚联合左旋多巴具有一定提高PD模型大鼠皮质中BBB通透性的作用。Objective To study the effect of the effective components of volatile oil of Acorus atarinowii Schott and levodopa（ L-dopa） co-administration on blood brain barrier in 6-hydroxydopamine induced Parkinson’s rat. Methods Twenty-four μg 6-OHDA was then injected into medial forebrain bundle（ MFB） of each rat at the following： antero-posterior：-4. 4 mm,medio-lateral：-1. 2 mm,dorso-ventral：-7. 8 mm. One month after the injection,seventy PD rats were successfully chosen according to apomorphine（ 0. 5 mg/kg,i. p.） ratation test（ 7 circles/min）. The PD rats were divided into four groups（ n = 10） ： model group,L-dopa group,β-asarone group,L-dopa ＋ β-asarone group. All the treated-rats were given by intragastric administration（ 5 m L/kg） twice a day for consecutive 30 days. Then levodopa and dopamine levels were determined by high performance liquid chromatography（ HPLC）. Zonula occludens-1（ ZO-1）,claudin-5,occludin and actin expressions in prefrontal cortex were detected by flow cytometry. Claudin-5 expression was detected by immunohistochemistry. Results Compared with control group,model group rats levodopa and dopamine levels were significantly reduced（ P 〈 0. 05）,the expressions of ZO-1,Occludin,Actin and Claudin-5 had no significant change（ P 〉 0. 05）. Compared with model group,combination group rats levodopa and dopamine levels were significantly increased（ P 〈 0. 01）,the expression of ZO-1,Occludin,Actin and Claudin-5 were significantly reduced（ P 〈 0. 05）. Compared with levodopa group,combination group rats levodopa and dopamine levels increased significantly（ P 〈 0. 05）,the expression of ZO-1,Occludin,Actin and Claudin-5 were significantly reduced（ P 〈 0. 05）. Conclusion Co-administration of β-asarone in effective components of volatile oil of Acorus tatarinowii Schott and levodopa has an effect on permeability of blood brain barrier in 6-hydroxydopamine induced Parkinson’s rat.

关 键 词：石菖蒲挥发油 左旋多巴 帕金森病模型 血脑屏障 

分 类 号：R285.5[医药卫生—中药学]