人参皂甙Rh2抗海马脑片H_2O_2损伤作用及与GABAA受体的关系  

The protective effect of ginsenoside Rh2 on H_2O_2 injury in hippocampal slices and the role of GABAA receptor

作　　者：江山[1] 陈莺 房德芳[1] 

机构地区：[1]江苏联合职业技术学院连云港中医药分院 [2]徐州医科大学附属连云港医院麻醉科,连云港222007

出　　处：《中药药理与临床》2016年第6期38-42,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：连云港市卫生局科研课题资助项目(编号:1218)

摘　　要：目的:研究人参皂甙Rh2对H_2O_2损伤保护作用及与GABAA受体的关系。方法:采用小鼠制备离体海马脑片,随机分为正常组、模型组、GABA组、人参皂甙Rh2(20μmol/L、40μmol/L、60μmol/L)组、荷包牡丹碱组、荷包牡丹碱+人参皂甙Rh2组,每组20张脑片。采用碘化丙啶(PI)染色法测定H_2O_2损伤后海马CA1区细胞活力变化,免疫组化方法观察细胞色素C表达,电生理技术记录顺向群峰电位(OPS)观察突触传递变化。结果:人参皂甙Rh2(40μmol/L、60μmol/L)可明显提高H_2O_2损伤后海马CA1区细胞活力,降低海马CA1区细胞色素C表达,海马脑片OPS恢复率和OPS恢复程度亦明显提高。人参皂甙Rh2的保护作用和外源性GABA作用相似并可被GABAA受体抑制剂荷包牡丹碱所阻断。结论:人参皂甙Rh2可减轻H_2O_2对海马CA1区细胞损伤,促进神经元突触传递功能的恢复,其作用机制与激活GABAA受体而减少细胞色素C的释放有关。Aim：To investigate the protective effect of ginsenoside Rh2 on H2O2 injury in hippocampal slices and therole of GABAA receptor.Methods：Mouse hippocampal slices were randomly divided into normal group, model group, GABA group, ginsenoside Rh2 （20μmol/L, 40μmol/L,60μ mol/L） group, bicuculline group and bicuculline ＋ ginsenoside Rh2 group. The cellular viability after H2O2 injury was deter-mined by propidium iodide （ PI ） staining, the changes of orthodromic population spike （ OPS ） were observed by electrophysiological technique, the expressions of cytochrome C in hippocampal CA1 regions were measured by immunohistoehemical assay. Results：Ginsenoside Rh2 （ 40μmol/L and 60μmol/L） improved significantly the recovery rate and amplitude of OPS ,which also significantly increased the cellular viability and significantly decreased the expression of cytochrome C in hippocampal CA1 regions after H2O2 injury. The effect of ginsenoside Rh2 was similar to exogenous GABA and reduced by GABAA receptor antagonist bicuculline. Conclusion：Ginsenoside Rh2 can antagonize H2O2 injury and improve the recovery of synaptic transmissioni neurons. The mechanism is related to activation of GABAA receptor and reduction of cytochrome C release.

关 键 词：人参皂甙RH2 H2O2 顺向群峰电位 细胞色素C GABA 

分 类 号：R285.5[医药卫生—中药学]