益气活血通络方对db/db小鼠胰腺组织JNK信号通路及IL-1β、TNF-α表达的影响  

Effect of Yiqihuoxuetongluo Recipe on JNK signal pathway and expression of IL-1β and TNF-α of pancreas in db/db mice

作　　者：尤良震[1] 申国明[2] 姚永传 王柳[1] 王芹[1] 何莹[1] 叶树[1] 江爱娟[2] 王浩[1] 

机构地区：[1]安徽中医药大学中西医结合临床学院,合肥230038 [2]安徽中医药大学大学研究生院,合肥230038

出　　处：《中药药理与临床》2016年第6期166-171,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(No.81273646);安徽中医药大学研究生科技创新基金

摘　　要：目的:观察益气活血通络方对db/db小鼠胰腺组织c-Jun氨基末端激酶(JNK)信号通路及炎症因子表达的影响,探讨益气活血通络方对胰岛细胞炎性损伤的保护作用及其机制。方法:用自发性2型糖尿病C57BL/Ks J-db/db小鼠为动物模型,将db/db小鼠随机分成模型组、硫辛酸组(0.078g/kg)、益气活血通络方6.4、3.2、1.6g/kg剂量组,另取10只db/m小鼠为正常对照组,连续灌胃给药8周,模型组给予生理盐水。药前、药后4周和8周测定小鼠血糖。实验结束后,摘取胰腺组织,HE染色法观察病理变化,ELISA法检测小鼠胰腺组织胰岛素含量,Western bloting与免疫组织化学法检测IL-1β、TNF-α、JNK、p-JNK蛋白的表达。结果:与正常组比较,模型组胰岛细胞出现严重的病理性损伤,药后4周和8周血糖、胰腺组织中IL-1β、TNF-α、P-JNK蛋白的表达水平明显高于正常组;与模型组比较,益气活血通络方组胰岛细胞病理损伤程度减轻,血糖降低,胰腺组织胰岛素含量升高,胰腺组织IL-1β、TNF-α、P-JNK蛋白表达水平显著下降。结论:益气活血通络方能够减轻db/db小鼠胰岛细胞损伤,可能与降低炎症因子表达,抑制JNK信号通路过度激活有关。Objective： To explore the effects of Yiqihuoxuetongluo Recipe（ YQHXTLR）, a compound triditional herbal medicine, on the JNK sig- naling pathway and the expression of IL-1β and TNF-α in pancreatic tissues of db/db mice to investigate the possible mechanisms of YQHX- TLR against islet cells damages. Methods： The experimental animals model of this study were C57 BL/KsJ-db/db mice which were spontane- ous type 2 diabetes animal model. The db/db mice were randomly divided into model group, lipoic acid（0.078g/kg）group and YQHXTLR groups （6.4,3.2, 1.6g/kg） and the db/m mice were used as normal group, 10 mice each group. Medication groups were treated with drug every day for 8 weeks. Model group mice were given normal saline. The blood glucose of 0,4,8 weeks were measured. After the end of the experiment, the pancreatic tissues were removed. The pancreatic island lesions were observed under light microscope by HE colouring method, the insulin secretion of pancreas were observed by ELISA method and the expression of IL-1β, TNF-α, JNK, P-JNK of pancreas were observed by Western bloting and Immunohisto chemistry methods. Results：Compared with normal group, model group islet cells appear serious pathological damage. The expression levels of IL-1β, TNF-α, P-JNK protein in pancreatic tissues and the blood glucose of 4,8 weeks were significantly higher in the model group than the normal group and the insulin secretion in pancreatic tissues were significantly lower. Compared with model group, YQHXTLR groups could reduce the damage of islet cells. The insulin secretion of YQHXTLR groups were high-r than in the model groupd , the expression levels of IL-1β, TNF-α, P-JNK protein and the blood glucose of 4,8 weeks were lower than in the model group. Conclusion： YQHXTLR could reduce the db/db mice islet cell damage, which might be reduced expression of inflammation factors and controlled excessive activation of JNK signaling pathway.

关 键 词：益气活血通络 胰岛细胞 JNK 信号通路 炎症因子 DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]