芪红脑苏汤对局灶性脑缺血再灌注损伤的保护机制研究  

The research of protective mechanism of Qihongnaosu decoction on rats with focal cerebral ischemia-reperfusion injury

作　　者：谢淑玲[1] 廖菁[2] 范子煊[3] 何林[2] 钟海洛[3] 伍文彬[1,4] 

机构地区：[1]成都中医药大学,成都610075 [2]四川省人民医院,成都610072 [3]四川省肿瘤医院,成都610041 [4]成都中医药大学附属医院,成都610075

出　　处：《中药药理与临床》2016年第6期171-175,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省科技厅应用基础项目(编号:2011JY0068);成都市科技计划项目(编号:12PPYB215SF-002)

摘　　要：目的:研究芪红脑苏汤活血化瘀作用对局灶性脑缺血再灌注损伤大鼠的脑保护作用,并探讨其作用机制。方法:以线栓法复制局灶性脑缺血再灌注损伤大鼠模型,观察芪红脑苏汤对局灶性脑缺血再灌注损伤大鼠脑组织病理形态改变及脑组织中TNF-α、ICAM-1含量的影响。结果:与模型组比较,芪红脑苏汤31.4g/kg、15.7g/kg、7.9g/kg剂量组均能降低大鼠神经功能缺失评分及其病理评分分值;芪红脑苏汤31.4g/kg剂量组能降低缺血部位TNF-α表达,31.4g/kg、15.7g/kg剂量组能降低缺血部位ICAM-1的表达;芪红脑苏汤31.4g/kg、15.7g/kg、7.9g/kg剂量组均能降低缺血部位TNF-α含量,15.7g/kg、7.9g/kg剂量组能降低缺血部位ICAM-1的含量。结论:芪红脑苏汤的活血化瘀作用对局灶性脑缺血再灌注大鼠的脑损伤具有较好的保护作用,其作用机制可能与降低脑内TNF-α、ICAM-1含量有关。Objective：To investigated the protective effect and mechanism of Qihongnaosu decoction on rats with transient focal cerebral ischemia- reperfusion injury. Methods：Rat models of transient focal cerebral ischemia-reperfusion injury were established by suture occlusion method, and then to observe the pathological changes and expressions of TNF-α and ICAM-1 in brain tissue. Results：Qihongnaosu-decoction of 31. 4g/kg, 15.7g/kg,7.9g/kg can reduce the neurological functional deficit scores and pathological scores of rats. Qihongnaosu-decoction of 31. 4g/kg can reduce the expression of TNF-α and 31.4g/kg,15.7g/kg can reduce the expression of ICAM-1 in ischemia area. Qihongnaosu decoction of 31.4g/kg, 15.7g/kg,7.9g/kg can reduce the content of TNF-α and 15.7g/kg,7.9g/kg can reduce the content of ICAM-1 in is- chemia area. Conelusio：Qihongnaosu decoction may reduce the damage after cerebral isehemia and has neuroproteetive effects on brain. Its mechanism may be related to the reduction of TNF-α and ICAM-1 in brain.

关 键 词：芪红脑苏汤 活血化瘀 局灶性脑缺血 肿瘤坏死因子-Α 细胞间粘附因子-1 

分 类 号：R285.5[医药卫生—中药学]