低频脉冲电磁场促进成骨细胞分化的基因调节和非基因调节探究  

Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation

作　　者：方清清 李志忠[2] 周建[1] 石文贵[1] 闫娟丽[1] 陈克明[1] 

机构地区：[1]兰州军区兰州总医院全军骨科中心骨科研究所,甘肃兰州730050 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《浙江大学学报（医学版）》2016年第6期568-574,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金(81270963,81471090)

摘　　要：目的：探究50Hz0．6mT脉冲电磁场（PEMF）促进体外培养大鼠颅骨成骨细胞（ROB）分化成熟是否存在基因调节和非基因调节。方法：体外培养ROB，传代融合后经50Hz0．6mTPEMF处理1．5h，于0、3、6、9、12h检测碱性磷酸酶（ALP）的活性及其基因表达，以及骨形成相关因子Runx2、OSX的基因和蛋白表达。结果：50Hz0．6mTPEMF处理后3h，ROB中ALP的活性高于对照组（P〈0．01），而其基因表达在处理后6h才高于对照组。Runx2的基因表达在处理后立即升高，持续至处理后6h回归对照组水平，之后再次升高，其蛋白表达与之相对应但略有滞后。OSX的基因表达亦在处理后立即升高，持续至处理后6h回归对照组水平，至12h时显著降低，其蛋白表达与之相对应但略有滞后。结论：50Hz0．6mTPEMF促进体外培养ROB分化成熟存在对Runx2和OSX的基因调节以及对ALP活性的非基因调节。Objective： To study the genic and non-genic regulation of 50 Hz 0. 6 mTpulsed electromagnenic fields （PEMF） on rat calvarial osteoblasts （ROB） differentiation. Methods： ROBs were achieved by enzyme digestion, and treated with 50 Hz 0. 6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase （ALP） activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment. Results： The ALP activity at 3 hours after treatment was significantly higher than that in the control （P 〈0.01 ）, while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay. Conclusions： There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0. 6 mT PEMFs prompts ROBs differentiation.

关 键 词：成骨细肜辐射效应 颅骨 电磁场 动物 新生 细胞 培养的 细胞 分化 基因 调节 大鼠 WISTAR 

分 类 号：R580[医药卫生—内分泌]