鼠尾Ⅰ型胶原的酸解、纤维重构和仿骨生物矿化研究  

Study on the acid hydrolysis,fiber remodeling and bionics mineralization of rat tail tendon collagen type Ⅰ

作　　者：张展[1] 张春[1] 郭峭峰[1] 

机构地区：[1]浙江省立同德医院骨科

出　　处：《浙江大学学报（医学版）》2016年第6期592-597,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科学技术厅科技计划(2013F50006);浙江省科学技术厅公益技术研究社会发展项目(2014C33207);浙江省医药卫生科技计划(2014KYA030)

摘　　要：目的：利用鼠尾Ⅰ型胶原构建与人类自体骨组织化学成分和分子结构相近的仿生骨材料。方法：通过醋酸酸解提取鼠尾Ⅰ型胶原蛋白，并重构成胶原纤维。然后，将重构后的胶原纤维放置在矿化液中模仿骨生物矿化2—6d，通过透射电子显微镜和电子衍射观察骨生物矿化。结果：酸解提取的鼠尾Ⅰ型胶原蛋白能够重构成胶原纤维，并且具有特征性的D-Band结构。透射电子显微镜和电子衍射显示：矿化2d后，羟基磷灰石钙前体渗入胶原纤维，胶原纤维部分矿化；矿化6d后，胶原纤维内部完全矿化，形成Ⅰ型胶原蛋白／羟基磷灰石钙的仿生骨材料。结论：利用酸解法提取的鼠尾Ⅰ型胶原蛋白可以重构成胶原纤维，经矿化可形成与人类自体骨组织化学成分和分子结构一致的仿生骨材料。Objective： To produce bionic bone material that is consistent with human bone in chemical composition and molecular structure using rat tail tendon collagen type Ⅰ . Methods： The type Ⅰcollagen derived from rat tail was extracted by acetic acid to form collagen fibers. The reconstructed collagen fibers were placed in the mineralized solution to mimic bone mineralization for 2 -6 days. Bone mineralization was observed by transmission electron microscopy and electron diffraction. Results：Collagen fibers with characteristic D-Band structure were reconstructed by using rat tail tendon collagen type Ⅰ extracted with acid hydrolysis method. Transmission electron microscopy and electron diffraction showed that calcium hydroxyapatite precursor infiltrated into the collagen fibers, and the collagen fibers were partially mineralized after 2 days of mineralization; the collagen fibers were completely mineralized and bionic bone material of type I collagen/calcium hydroxyapatite was formed after 6 days of mineralization. Conclusion： The collagen type Ⅰ can be extracted from rat tail tendon by acid hydrolysis method, and can be reformed and mineralized to form the bionic bone material which mimics human bone in chemical composition and the molecular structure.

关 键 词：胶原Ⅰ型 纤维胶原类 羟基磷灰石类 生物相容性材料 大鼠 尾 

分 类 号：R318.08[医药卫生—生物医学工程]